Lipopolysaccharide induced MAP kinase activation in RAW 264.7cells attenuated by cerium oxide nanoparticles

Details

• Personal Author:
  Arvapalli R ; Asano S ; Blough ER ; Fankenhanel E ; Ma JY ; Maheshwari M ; Manne NDPK ; Nepal N ; Rice KM ; Rogers S ; Selvaraj V ; Shokuhfar T
• Description:
  High mortality rates are associated with the life threatening disease of sepsis. Improvements in septic patient survivability have failed to materialize with currently available treatments. This article represents data regarding a study published in biomaterials (Vellaisamy et al., Biomaterials, 2015, in press) with the purpose of evaluating whether severe sepsis mortality and associated hepatic dysfunction induced by lipopolysaccharide (LPS) can be prevented by cerium oxide nanoparticles (CeO2NPs) treatment in male Sprague Dawley rats. Here we provide the information about the method and processing of raw data related to our study publish in Biomaterials and Data in Brief (Vellaisamy et al., Biomaterials, 2015, in press; Vellaisamy et al., Data in Brief, 2015, in press.). The data contained in this article evaluates the contribution of MAPK signaling in LPS induced sepsis. Macrophage cells (RAW 264.7) were treated with a range of cerium oxide nanoparticle concentration in the presence and absence of LPS. Immunoblotting was performed on the cell lysates to evaluate the effect of cerium oxide nanoparticle treatment on LPS induced changes in Mitogen Activated Protein Kinases (MAPK) p-38, ERK 1/2, and SAPK/JNK phosphorylation. [Description provided by NIOSH]
• Subjects:
  Animals Biological Products Blood Laboratories Morbidity Mortality Occupational Health Safety
• Keywords:
  Author Keywords: Sepsis Biological-material Blood-cells Cerium-compounds Diseases In-vivo-study Laboratory-animals LPS MAPK Morbidity-rates Mortality-rates Nanotechnology Physiological-factors Physiology Proteins Raw 264.7 Cells Survival-rate

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• ISSN:
  2352-3409
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Michigan ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  96-99
• Volume:
  4
• NIOSHTIC Number:
  nn:20046568
• Citation:
  Data Brief 2015 Sep; 4:96-99
• Contact Point Address:
  E.R. Blough, Center for Diagnostic Nanosystems, Robert C. Byrd Biotechnology Science Center, Marshall University, 1700 3rd Ave., Huntington, WV 25755-1090 USA
• Email:
  blough@marshall.edu
• CAS Registry Number:
  Ceria (CAS RN 1306-38-3)
• Federal Fiscal Year:
  2015
• Peer Reviewed:
  True
• Source Full Name:
  Data in Brief
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:54151d82b279fcfe2a2e7fcb02878cfa18d946c76115aa07ac4aade0b52588ff9ee2b5990a2dfb849ce465e205a4c1a9f8d8aca5a09c76e36a6e09f162845f42
• Download URL:
  https://stacks.cdc.gov/view/cdc/201582/cdc_201582_DS1.pdf
• File Type:
  [PDF - 307.56 KB ]