Association of airborne virus infectivity and survivability with its carrier particle size

Details

• Personal Author:
  Appert J ; Ge S ; Goyal SM ; Kuehn TH ; Kumar S ; Pui DYH ; Raynor PC ; Verma H ; Zuo Z
• Description:
  Although laboratory generated virus aerosols have been widely studied in terms of infectivity and survivability, how they are related to particle size, especially in the submicron size range, is little understood. Four viruses (MS2 bacteriophage, transmissible gastroenteritis virus, swine influenza virus, and avian influenza virus) were aerosolized, size classified (100-450 nm) using a differential mobility analyzer (DMA), and collected onto gelatin filters. Uranine dye was also nebulized with the virus, serving as a particle tracer. Virus infectivity assay and quantitative reverse transcription-polymerase chain reaction were then used to quantify the amount of infectious virus and total virus present in the samples, respectively. The virus distribution was found to be better represented by the particle volume distribution rather than the particle number distribution. The capacity for a particle to carry virus increased with the particle size and the relationship could be described by a power law. Virus survivability was dependent on virus type and particle size. Survivability of the three animal viruses at large particle size (300-450 nm) was significantly higher than at particle size close to the size of the virion (100-200 nm), which could be due to the shielding effect. The data suggest that particle size plays an important role in infectivity and survivability of airborne viruses and may, therefore, have an impact on the airborne transmission of viral illness and disease. The data in this study do not support the use of MS2 bacteriophage as a general surrogate for animal and human viruses. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Communicable Diseases Laboratories Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety Viral Diseases
• Keywords:
  Airborne-particles Filters Infection-control Infectious-diseases Laboratory-testing Particulates Respiration Respiratory-system-disorders Sampling Sampling-equipment Viral-diseases Viral-infections

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• ISSN:
  0278-6826
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Minnesota ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  373-382
• Volume:
  47
• Issue:
  4
• NIOSHTIC Number:
  nn:20046478
• Citation:
  Aerosol Sci Technol 2013 Apr; 47(4):373-382
• Contact Point Address:
  Zhili Zuo, Department of Mechanical Engineering, University of Minnesota, 111 Church Street SE, Minneapolis, MN 55455, USA
• Email:
  zuoxx011@umn.edu
• Federal Fiscal Year:
  2013
• Performing Organization:
  University of Minnesota, Schools of Veterinary Medicine, Minneapolis, Minnesota
• Peer Reviewed:
  True
• Start Date:
  20080901
• Source Full Name:
  Aerosol Science and Technology
• End Date:
  20130831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:64a21ea8b6c5557287b60e6810df0400418b79b804c584aaae19acaf8b1750dd99daf79a45a7a3ef9fb7193d0bac9e9b5d95e5bda73efd4a555a2c1edd8c4bd2
• Download URL:
  https://stacks.cdc.gov/view/cdc/201526/cdc_201526_DS1.pdf
• File Type:
  [PDF - 352.98 KB ]