Airborne virus survivability during long-term sampling using a non-viable Andersen cascade impactor in an environmental chamber

Details

• Personal Author:
  Abin M ; Appert J ; Bekele A ; Ge S ; Goyal SM ; Kuehn TH ; Mor SK ; Raynor PC ; Verma H ; Zuo Z
• Description:
  In order to evaluate the survivability of airborne viruses and the sampling performance of an eight-stage non-viable Andersen impactor in typical indoor environments featuring low viral aerosol concentrations, aerosols of a male-specific bacteriophage (MS2), human adenovirus type 1 (HAdV-1), and avian influenza virus (AIV) were sampled size-selectively using the impactor in an environmental chamber. Live virus titer, total virus RNA or DNA concentration, and intensity of a fluorescein tracer were measured to calculate relative virus recovery and virus survival. Viral aerosols were first sampled for 1 and 6 h at 25 degrees C and 50% relative humidity (RH). Virus inactivation and plate overloading were found to be significant in the impactor. Viral aerosols were then sampled at different temperature and humidity levels. MS2 and AIV showed higher survival at lower temperature. Absolute humidity (AH) was found to be a better predictor of virus survival than RH, and the interaction between AH and temperature was not significant. For the tested AH range of 8.8 to 15.2 g/m3, MS2 and HAdV-1 had the highest survival at the lowest AH while AIV had the highest survival at the highest AH. More than 95% of mass collected was for particles smaller than 4.7 mm, with the mass median diameter of 1.5 mm. In the nebulizer, virus inactivation was not significant at 10 psi (69 kPa) compressed air pressure for up to 6 h of nebulization. Nebulizer analysis also reveals that the use of fluorescein tracer may not always accurately predict the physical loss of virus. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Communicable Diseases Humidity Laboratories Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety Temperature Viral Diseases

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• Keywords:
  Airborne-particles Filters Infection-control Infectious-diseases Laboratory-testing Particulates Respiration Respiratory-system-disorders Sampling Sampling-equipment Temperature-effects Viral-diseases Viral-infections

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• ISSN:
  0278-6826
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Minnesota ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  48
• Issue:
  12
• NIOSHTIC Number:
  nn:20046479
• Citation:
  Aerosol Sci Technol 2014 Dec; 48(12):1360-1368
• Contact Point Address:
  Song Ge, Department of Mechanical Engineering, College of Science and Engineering, University of Minnesota, 111 Church Street SE, Minneapolis, MN 55455
• Email:
  ges@umn.edu
• Federal Fiscal Year:
  2015
• Performing Organization:
  University of Minnesota, Schools of Veterinary Medicine, Minneapolis, Minnesota
• Peer Reviewed:
  True
• Start Date:
  20080901
• Source Full Name:
  Aerosol Science and Technology
• End Date:
  20130831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3c29929f680172a75893c5545f940e84118036345af846701d78abd62ac4fef5fa65de47830cae30e79884a48d380c69103ccedc641994db5a9fbb5bbabf3369
• Download URL:
  https://stacks.cdc.gov/view/cdc/201527/cdc_201527_DS1.pdf
• File Type:
  [PDF - 320.46 KB ]