Comparison of spike and aerosol challenge tests for the recovery of viable influenza virus from non-woven fabrics

Details

• Personal Author:
  Chander Y ; de Abin M ; Goyal SM ; Kuehn TH ; Pui DYH ; Zuo Z
• Description:
  Background: To experimentally determine the survival kinetics of influenza virus on personal protective equipment (PPE) and to evaluate the risk of virus transfer from PPE, it is important to compare the effects on virus recovery of the method used to contaminate the PPE with virus and the type of eluent used to recover it. Methods: Avian influenza virus (AIV) was applied as a liquid suspension (spike test) and as an aerosol to three types of non-woven fabrics [polypropylene (PP), polyester (PET), and polyamide (Nylon)] that are commonly used in the manufacture of PPE. This was followed by virus recovery using eight different eluents (phosphate-buffered saline, minimum essential medium, and 1·5% or 3·0% beef extract at pH 7, 8, or 9). Results: For spike tests, no statistically significant difference was found in virus recovery using any of the eluents tested. Hydrophobic surfaces (PP and PET) yielded higher spiked virus recovery than hydrophilic Nylon. From all materials, the virus recovery was much lower in aerosol challenge tests than in spike tests. Conclusions: Significant differences were found in the recovery of viable AIV from non-woven fabrics between spike and aerosol challenge tests. The findings of this study demonstrate the need for realistic aerosol challenge tests rather than liquid spike tests in studies of virus survival on surfaces where airborne transmission of influenza virus may get involved. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollutants, Occupational Occupational Health Particulate Matter Personal Protective Equipment Risk Assessment Risk Factors Safety Viral Diseases
• Keywords:
  Airborne-particles Author Keywords: Aerosol Exposure-levels Influenza Virus Non-woven Fabric Personal-protection Personal-protective-equipment Personal Protective Equipment Risk-factors Spike Viral-diseases Viral-infections

  More +
• ISSN:
  1750-2640
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Minnesota ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  7
• Issue:
  5
• NIOSHTIC Number:
  nn:20046477
• Citation:
  Influenza Other Respir Viruses 2013 Sep; 7(5):637-644
• Contact Point Address:
  Zhili Zuo, Department of Mechanical Engineering, University of Minnesota, 111 Church Street SE, Minneapolis, MN 55455, USA
• Email:
  zuoxx011@umn.edu
• Federal Fiscal Year:
  2013
• Performing Organization:
  University of Minnesota, Schools of Veterinary Medicine, Minneapolis, Minnesota
• Peer Reviewed:
  True
• Start Date:
  20080901
• Source Full Name:
  Influenza and Other Respiratory Viruses
• End Date:
  20130831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0fc7909beb7b30323d4c3b7ea0938c6e1ad4b2938bb04182c331b6ce5528388301685dc99cc3a549d2e40bb77f5eb71fa96a8b0966f95810e5667bb671968766
• Download URL:
  https://stacks.cdc.gov/view/cdc/201525/cdc_201525_DS1.pdf
• File Type:
  [PDF - 207.26 KB ]