Comparison of eluent and aerosol vs. liquid spide challenge tests for influenza virus recovery from npn-woven fabrics

Details

• Personal Author:
  Abin M ; Chander Y ; Kuehn T ; Zuo Z
• Description:
  Seasonal influenza in the U.S. annually causes >200,000 hospitalizations and approximately 36000 deaths. One way to reduce the spread of influenza is to use personal protective equipment (PPE). Previous studies which used liquid spike tests have found that influenza virus could survive on PPE and virus could transfer from contaminated PPE to human hands. To better understand influenza survival on PPE and assess the risk of influenza virus transfer from contaminated PPE, there is a need for determining the optimum eluent for recovering influenza from PPE by comparing the recovery efficiency of different eluents. In addition, the relative contribution for influenza transmission by large droplets and smaller aerosol particles is controversial. Virus recovery may be different when the virus is applied as a liquid suspension vs. an aerosol. In this study, eight eluents (phosphate buffer saline (PBS), minimum essential medium (MEM), and beef extract (BE) of two different concentrations (1.5% and 3.0%) at three different pH values (7.0, 8.0, and 9.0)) were evaluated for their recovery efficiency of avian influenza virus H9N9 from three non-woven fabrics (polypropylene, polyester, and Nylon) that are commonly used to manufacture PPE. Spike tests followed by elution at three drying times (immediately after applying the virus suspension, the time when the applied virus became air-dried, and 30 min after the virus dried) showed no significant difference in the recovery efficiencies of the eleunts (p = 0.13). However, the type of non-woven fabric significantly affected the recovery (p < 0.000), with the largest from PP and the smallest from Nylon. In addition, recovery efficiency generally decreased with increased drying time (p < 0.000), due to virus desiccation. Preliminary aerosol challenge tests using both virus infectivity assay and uranine tracer showed the recovery was lower than for the spike tests. Detailed comparison of recovery between the spike tests and aerosol tests are presented. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollutants, Occupational Communicable Diseases Men Occupational Health Particulate Matter Personal Protective Equipment Public Health Safety Viral Diseases Women

  More +
• Keywords:
  Aerosol-particles Airborne-particles Humans Infectious-diseases Personal-protection Personal-protective-equipment Public-health Viral-diseases Viral-infections
• Publisher:
  American Association for Aerosol Research
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Minnesota ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20046488
• Citation:
  Proceedings of the AAAR 30th Annual Conference, October 4-7, 2011, Orlando, Florida. Mount Laurel, NJ: American Association for Aerosol Research, 2011 Oct; :2C.8
• Federal Fiscal Year:
  2012
• Performing Organization:
  University of Minnesota, Schools of Veterinary Medicine, Minneapolis, Minnesota
• Peer Reviewed:
  False
• Start Date:
  20080901
• Source Full Name:
  Proceedings of the AAAR 30th Annual Conference, October 4-7, 2011, Orlando, Florida
• End Date:
  20120831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9bb1948bdc52e2f024117b01c12e982fe8f0880885ca1fb9e333fe4a88135c9bab58018c95648273c5581707fedcc6b2e604da3d9f2ce95ffc7ec50492c1db56
• Download URL:
  https://stacks.cdc.gov/view/cdc/201536/cdc_201536_DS1.pdf
• File Type:
  [PDF - 89.72 KB ]