Influence of suspending liquid, impactor type, and substrate on size-selective sampling of MS2 and adenovirus aerosols

Details

• Personal Author:
  Abin M ; Appert J ; Chander Y ; Ge S ; Goyal SM ; Guarino H ; Kuehn TH ; Raynor PC ; Zuo Z ; Abin M ; Appert J ; Chander Y ; Ge S ; Goyal SM ; Guarino H ; Kuehn TH ; Raynor PC ; Zuo Z
• Description:
  Size-selective sampling methods for detecting viral aerosols are needed to assess the risk of airborne transmission of disease. Andersen and MOUDI nonviable cascade impactors were used to separate test aerosols containing MS2 bacteriophage or adenovirus into size fractions spanning much of the human respirable range. Culture-based methods and a fluorescent tracer dye allowed quantification of the viral particles that remained infective after being aerosolized into a test apparatus and collected by the impactors. In addition, various suspension fluids and impaction surfaces were evaluated for their effect on virus viability. Both the Andersen and MOUDI impactors were able to sample live viruses from test aerosols, although the relative recovery rate of MS2 was higher than adenovirus (P < 0.001). The MS2 and adenovirus aerosols were sensitive to different test factors. MS2 recovery was dependent on the suspension fluid (P < 0.0001) and RH (P = 0.001), whereas adenovirus recovery was dependent on aerodynamic particle size (P < 0.001). Relative recovery of adenovirus was highest in the 0.56-1.9 um diameter range. The results confirm that nonviable cascade impactors are capable of size-separating and detecting aerosolized viruses in the human respirable range, and that MS2 and adenovirus can retain viability after nebulization under experimental conditions. The findings cast doubt, however, on the suitability of MS2 as a general surrogate for human and animal viruses. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollutants, Occupational Occupational Health Particulate Matter Personal Protective Equipment Respiratory Protective Devices Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety Viral Diseases

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• Keywords:
  Airborne-particles Diseases Respiration Respiratory-infections Respiratory-protection Respiratory-system-disorders Risk-factors Sampling Sampling-methods Viral-diseases Viral-infections

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• ISSN:
  0278-6826
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  Minnesota ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Pages in Document:
  249-257
• Volume:
  46
• Issue:
  3
• NIOSHTIC Number:
  nn:20046475
• Citation:
  Aerosol Sci Technol 2012 Mar; 46(3):249-257
• Contact Point Address:
  Jessica Appert, Division of Environmental Health Sciences, School of Public Health, University of Minnesota, 1260 Mayo (MMC 807), 420 Delaware Street SE, Minneapolis, MN 55455
• Email:
  appert@umn.edu
• Federal Fiscal Year:
  2012
• Performing Organization:
  University of Minnesota, Schools of Veterinary Medicine, Minneapolis, Minnesota
• Peer Reviewed:
  True
• Start Date:
  20080901
• Source Full Name:
  Aerosol Science and Technology
• End Date:
  20130831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b3f1dc708d0fc20b0ec97da35ab43ae2c193815e6a817cd6c24323e5c9cec554ae67a4cbb50f9fd5151bacd62c45ead961973c68f4a3bba69e5c59d3b6ff17c9
• Download URL:
  https://stacks.cdc.gov/view/cdc/201523/cdc_201523_DS1.pdf
• File Type:
  [PDF - 322.31 KB ]