Effects of nitrogen-doped multi-walled carbon nanotubes compared to pristine multi-walled carbon nanotubes on human small airway epithelial cells

Details

• Personal Author:
  Castranova, Vincent ; Cruz-Silva R ; Ding W ; Endo M ; McLoughlin C ; Mihalchik AL ; Porter DW ; Qian Y ; Schwegler-Berry-D ; Sisler JD ; Snyder-Talkington BN ; Stefaniak, Aleksandr B. ; Terrones M ; Tsuruoka S
• Description:
  Nitrogen-doped multi-walled carbon nanotubes (ND-MWCNTs) are modified multi-walled carbon nanotubes (MWCNTs) with enhanced electrical properties that are used in a variety of applications, including fuel cells and sensors; however, the mode of toxic action of ND-MWCNT has yet to be fully elucidated. In the present study, we compared the interaction of ND-MWCNT or pristine MWCNT-7 with human small airway epithelial cells (SAEC) and evaluated their subsequent bioactive effects. Transmission electron microscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, and X-ray diffraction suggested the presence of N-containing defects in the lattice of the nanotube. The ND-MWCNTs were determined to be 93.3% carbon, 3.8% oxygen, and 2.9% nitrogen. A dose-response cell proliferation assay showed that low doses of ND-MWCNT (1.2 ug/ml) or MWCNT-7 (0.12 mg/ml) increased cellular proliferation, while the highest dose of 120 mg/ml of either material decreased proliferation. ND-MWCNT and MWCNT-7 appeared to interact with SAEC at 6 h and were internalized by 24 h. ROS were elevated at 6 and 24 h in ND-MWCNT exposed cells, but only at 6 h in MWCNT-7 exposed cells. Significant alterations to the cell cycle were observed in SAEC exposed to either 1.2 mg/ml of ND-MWCNT or MWCNT-7 in a time and material-dependent manner, possibly suggesting potential damage or alterations to cell cycle machinery. Our results indicate that ND-MWCNT induce effects in SAEC over a time and dose-related manner which differ from MWCNT-7. Therefore, the physicochemical characteristics of the materials appear to alter their biological effects. [Description provided by NIOSH]
• Subjects:
  Antioxidants Electricity Hazardous Substances Nitrogen Compounds Occupational Health Safety
• Keywords:
  Author Keywords: Multi-walled Carbon Nanotubes Biological-effects Carbon-compounds Cell-biology Cell-function Cellular-function Electrical-properties Functionalized Multi-walled Carbon Microscopy Nanotechnology Nanotubes Nitrogen-compounds Reactive Oxygen Species Toxins

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• ISSN:
  0300-483X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  25-36
• Volume:
  333
• NIOSHTIC Number:
  nn:20046048
• Citation:
  Toxicology 2015 Jul; 333:25-36
• Contact Point Address:
  Yong Qian, NIOSH, HELD, PPRB, 1095 Willowdale Dr., Morgantown, WV 26505
• Email:
  yaq2@cdc.gov
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0) ; Nitrogen (CAS RN 7727-37-9) ; Oxygen (CAS RN 7782-44-7)
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8b0cf350336f439ced7b7dc880428f87df634665e3c3ef0774184274250ca4246b87fc3889b9324c482355c4df8257875160539a84720e04c904ce5bdebbee2a
• Download URL:
  https://stacks.cdc.gov/view/cdc/201232/cdc_201232_DS1.pdf
• File Type:
  [PDF - 2.45 MB ]