Nano-gold corking and enzymatic uncorking of carbon nanotube cups

Details

• Personal Author:
  Burkert SC ; Kagan VE ; Kapralov AA ; Shurin GV ; Shurin MR ; Sorescu DC ; Star A ; Tang Y ; Zhao Y
• Description:
  Because of their unique stacked, cup-shaped, hollow compartments, nitrogen-doped carbon nanotube cups (NCNCs) have promising potential as nanoscale containers. Individual NCNCs are isolated from their stacked structure through acid oxidation and subsequent probe-tip sonication. The NCNCs are then effectively corked with gold nanoparticles (GNPs) by sodium citrate reduction with chloroauric acid, forming graphitic nanocapsules with significant surface-enhanced Raman signature. Mechanistically, the growth of the GNP corks starts from the nucleation and welding of gold seeds on the open rims of NCNCs enriched with nitrogen functionalities, as confirmed by density functional theory calculations. A potent oxidizing enzyme of neutrophils, myeloperoxidase (MPO), can effectively open the corked NCNCs through GNP detachment, with subsequent complete enzymatic degradation of the graphitic shells. This controlled opening and degradation was further carried out in vitro with human neutrophils. Furthermore, the GNP-corked NCNCs were demonstrated to function as novel drug delivery carriers, capable of effective (i) delivery of paclitaxel to tumor-associated myeloid-derived suppressor cells (MDSC), (ii) MPO-regulated release, and (iii) blockade of MDSC immunosuppressive potential. [Description provided by NIOSH]
• Subjects:
  Animals Chemistry Energy Metabolism Laboratories Nitrogen Compounds Occupational Health Safety
• Keywords:
  Cell-function Cellular-function Cellular-reactions Chemical-properties Laboratory-animals Metabolism Models Nanotechnology Neutrophils Nitrogen-compounds Oxidation Sodium-compounds

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• ISSN:
  0002-7863
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  137
• Issue:
  2
• NIOSHTIC Number:
  nn:20046840
• Citation:
  J Am Chem Soc 2015 Jan; 137(2):675-684
• Contact Point Address:
  Alexander Star, Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260
• Email:
  astar@pitt.edu
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Journal of the American Chemical Society
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a09715a2c5a7feed901bb5078db6edb60894c60858e6069e6e839d2e30ea9985e4cfdf08a6b3471906743e07db53dcc7160c50dc920288e3767d1b74f515d148
• Download URL:
  https://stacks.cdc.gov/view/cdc/202130/cdc_202130_DS1.pdf
• File Type:
  [PDF - 5.40 MB ]