Generation of reactive oxygen species from silicon nanowires

Details

• Personal Author:
  Bangsaruntip S ; Cohen GM ; Fix NR ; Kenyon AJ ; Leonard, Stephen S. ; Roberts, Jennifer R. ; Schwegler-Berry D
• Description:
  Processing and synthesis of purified nanomaterials of diverse composition, size, and properties is an evolving process. Studies have demonstrated that some nanomaterials have potential toxic effects and have led to toxicity research focusing on nanotoxicology. About two million workers will be employed in the field of nanotechnology over the next 10 years. The unknown effects of nanomaterials create a need for research and development of techniques to identify possible toxicity. Through a cooperative effort between National Institute for Occupational Safety and Health and IBM to address possible occupational exposures, silicon-based nanowires (SiNWs) were obtained for our study. These SiNWs are anisotropic filamentary crystals of silicon, synthesized by the vapor-liquid-solid method and used in bio-sensors, gas sensors, and field effect transistors. Reactive oxygen species (ROS) can be generated when organisms are exposed to a material causing cellular responses, such as lipid peroxidation, H2O2 production, and DNA damage. SiNWs were assessed using three different in vitro environments (H2O2, RAW 264.7 cells, and rat alveolar macrophages) for ROS generation and possible toxicity identification. We used electron spin resonance, analysis of lipid peroxidation, measurement of H2O2 production, and the comet assay to assess generation of ROS from SiNW and define possible mechanisms. Our results demonstrate that SiNWs do not appear to be significant generators of free radicals. [Description provided by NIOSH]
• Subjects:
  Biological Assay DNA Environmental Monitoring Free Radicals Hazardous Substances Laboratories Lung Occupational Health Peroxides Respiratory System Safety Silicon Compounds Toxicology

  More +
• Keywords:
  Alveolar-cells Author Keywords: Reactive Oxygen Species Bioassays Cellular-reactions DNA-damage Exposure-assessment Free-radical-generation Free-radicals Free Radicals Health-protection In-vitro-study Laboratory-testing Lipid-peroxidation Medical-research Nanomaterials Nanotechnology Nanotoxicology Oxidative-processes Silicon Silicon-compounds Toxic-materials

  More +
• ISSN:
  1178-6302
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  21-29
• Volume:
  8
• NIOSHTIC Number:
  nn:20045363
• Citation:
  Environ Health Insights 2014 Nov; 8(Suppl 1):21-29
• Contact Point Address:
  Stephen S. Leonard, National Institute for Occupational Safety and Health, Pathology and Physiology Research Branch, Morgantown, WV, USA
• Email:
  SEL5@CDC.GOV
• CAS Registry Number:
  Hydrogen peroxide (CAS RN 7722-84-1) ; Silicon (CAS RN 7440-21-3)
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Environmental Health Insights
• Supplement:
  1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d16b156c3f7e5a6b6dde7f5f4df34b6398a1ccd48266fbda12fc92f10bde28b17fdf5fdfa31c3dc12a224c4dd0493ea04bd7d9c770fd303c70de49b0563efa9f
• Download URL:
  https://stacks.cdc.gov/view/cdc/200811/cdc_200811_DS1.pdf
• File Type:
  [PDF - 1.09 MB ]