Luciferase reporter cells as a platform to detect SMAD-dependent collagen production

Details

• Personal Author:
  Liu Y ; Lohcharoenkal W ; Rojanasakul Y ; Wang L ; Yang Y
• Description:
  Aberrant collagen production can lead to many diseases such as fibrosis. Current methods of collagen detection are insensitive, time-consuming and laborious. We have developed a rapid, sensitive assay using chemiluminescence-based reporter cell system. Stable 3T3/NIH-SMAD-luciferase cells were generated for detection of collagen expression through TGF-β signaling, a major fibrogenic pathway. We demonstrated that these reporter cells could be used as a rapid screening tool for detection of SMAD-dependent collagen production with higher sensitivity than existing assays. Flexibility of this cell-based assay in different detection platforms makes it attractive for high throughput screening of potential fibrogenic agents and drug candidates. [Description provided by NIOSH]
• Subjects:
  Biological Assay Cytology Fibrosis Occupational Health Safety
• Keywords:
  Author Keywords: Luciferase Bioassays Biochemical Indicators Cell Function Collagen Fibrogenicity Luminescence Reporter Cells Screening Methods TGF-β
• ISSN:
  1389-1723
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  118
• Issue:
  8
• NIOSHTIC Number:
  nn:20044667
• Citation:
  J Biosci Bioeng 2014 Dec; 118(8):732-735
• Contact Point Address:
  Yon Rojanasakul, West Virginia University, Department of Pharmaceutical Sciences, Mary Babb Randolph Cancer Center, Morgantown, WV 26506
• Email:
  yrojan@hsc.wvu.edu
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Bioscience and Bioengineering
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f6335d8ddcfa904fe99e91ab1cb6a2fd49591d4bf0ab2297eac777b1daed902e4ae84e4022156b4dc2debdf98a3361c7e8e35c02368fe7be2e0f0a1c399af2a6
• Download URL:
  https://stacks.cdc.gov/view/cdc/200318/cdc_200318_DS1.pdf
• File Type:
  [PDF - 970.21 KB ]