Development of a Bioassay for Pulmonary Cell Production of Fibrogenic Factors

Details

• Personal Author:
  Blackford J ; Bryner K ; Castranova, Vincent ; Dey R ; Reist R ; Vrana K ; Wearden P
• Description:
  Fibroblast proliferation and enhancement of collagen synthesis are key steps in the development and progression of pulmonary fibrosis. The current investigation presents a fibroblast proliferation assay to detect the release of competence factors from pneumocytes. The specific objective of this study was to define cell culture conditions for fibroblasts that would allow selective detection of competence factors in test media. The results indicate the following: (a) fibroblasts should be cultured for 2 days in plasma-free medium rather than 2% plasma to assure complete quiescence; (b) 1% plasma was optimum for producing progression activity for the bioassay of a competence factor, while 0.5% plasma did not produce sufficient progression activity (yielding false negatives) and 2% plasma often contained significant competence activity (yielding false positives); and (c) fresh plasma should be used since the progression activity of plasma was adversely affected by freezing and thawing. Under these conditions, this bioassay can be employed with either rat or human lung fibroblasts to detect competence factors, such as platelet-derived growth factor (PDGF), at levels as low as 25 ng/ml. [Description provided by NIOSH]
• Subjects:
  Biological Assay Fibrosis Occupational Health Respiratory Tract Diseases Safety Urogenital Diseases
• Keywords:
  Bioassays Fibrogenesis Fibrogenicity Pulmonary-system-disorders Reproductive-system-disorders
• ISSN:
  1051-7235
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  53-65
• Volume:
  1
• Issue:
  1
• NIOSHTIC Number:
  nn:10012236
• Citation:
  Toxicology Methods 1991 Jan; 1(1):53-65
• Contact Point Address:
  Dr. V. Castranova, Biochemistry Section, NIOSH, 944 Chestnut Ridge Road, MS 211, Morgantown, WV 26505, U.S.A.
• Federal Fiscal Year:
  1991
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology Methods
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2ad81daddb86d549c81d9fc6c47a5932839ac07ca292269d27bd0bfe8751f7cf2bcc897c0dcbf878c976a4094a9f130c204a3c1c9bc1a407c6062da2e8da4722
• Download URL:
  https://stacks.cdc.gov/view/cdc/198135/cdc_198135_DS1.pdf
• File Type:
  [PDF - 1017.65 KB ]