A survey of liquid chromatographic-mass spectrometric analysis of mercapturic acid biomarkers in occupational and environmental exposure monitoring

Details

• Personal Author:
  B'Hymer, Clayton ; Mathias PI
• Description:
  High-performance liquid chromatography/mass spectrometry (HPLC/MS) is sensitive and specific for targeted quantitative analysis and is readily utilized for small molecules from biological matrices. This brief review describes recent selected HPLC/MS methods for the determination of urinary mercapturic acids (mercapturates) which are useful as biomarkers in characterizing human exposure to electrophilic industrial chemicals in occupational and environmental studies. Electrophilic compounds owing to their reactivity are used in chemical and industrial processes. They are present in industrial emissions, are combustion products of fossil fuels, and are components in tobacco smoke. Their presence in both the industrial and general environments are of concern for human and environmental health. Urinary mercapturates which are the products of metabolic detoxification of reactive chemicals provide a non-invasive tool to investigate human exposure to electrophilic toxicants. Selected recent mercapturate quantification methods are summarized and specific cases are presented. The biological formation of mercapturates is introduced and their use as biomarkers of metabolic processing of electrophilic compounds is discussed. Also, the use of liquid chromatography/tandem mass spectrometry in simultaneous determinations of the mercapturates of multiple parent compounds in a single determination is considered, as well as future trends and limitations in this area of research. [Description provided by NIOSH]
• Subjects:
  Acids Biomarkers Chemistry Chromatography Environmental Exposure Laboratories Mass Spectrometry Occupational Exposure Occupational Health Safety Urine Workplace

  More +
• Keywords:
  Author Keywords: Liquid Chromatography Chemical-analysis Chromatographic-analysis Environmental-exposure Industrial Chemicals Laboratory-equipment Laboratory-techniques Liquid-chromatography Mass-spectrometry Mass Spectrometry Mercapturic Acid Occupational Exposure Quantitative-analysis Urinalysis Urinary Biomarker Workplace-monitoring

  More +
• ISSN:
  1570-0232
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  136-145
• Volume:
  964
• NIOSHTIC Number:
  nn:20044275
• Citation:
  J Chromatogr B 2014 Aug; 964:136-145
• Contact Point Address:
  Patricia I. Mathias, National Institute for Occupational Safety and Health, Divisionof Applied Science and Technology, Robert A. Taft Laboratories, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
• Email:
  pmathias@cdc.gov
• CAS Registry Number:
  N-Acetyl-L-cysteine (CAS RN 616-91-1)
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Services
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b6294b50f746e74e69d53f6e6811e60cf85d887d9cca993c654d5d3638463f202205ddf926c4a83d3b0917c74accf831ce03c2c7d0ec39a18e5db4f7fa8dbc37
• Download URL:
  https://stacks.cdc.gov/view/cdc/200013/cdc_200013_DS1.pdf
• File Type:
  [PDF - 602.01 KB ]