Direct analysis of trichloropyridinol in human saliva using an Au nanoparticles-based immunochromatographic test strip for biomonitoring of exposure to chlorpyrifos

Details

• Personal Author:
  Du D ; Lin Y ; Liu D ; Smith J ; Tang Y ; Timchalk C ; Zhang W
• Description:
  A portable immunochromatographic strip-based biosensor for direct detection of trichloropyridinol (TCP), a specific biomarker of exposure to chlorpyrifos, in human saliva sample is presented. In this approach, a series of immunoreactions was performed on the test strip, where the targeted analytes (TCP) bound to the Au nanoparticles-labeled antibodies on the conjugate pad to form analyte-Au-antibody conjugates, and then free Au-labeled antibodies were captured by TCP-BSA in the test zone. Captured Au nanoparticles, increased with decreased levels of analytes, can be observed visibly without any equipment and later quantified by a colorimetric reader. Several experimental parameters were optimized including Au nanoparticle-to-TCP antibody coupling ratio, the amount of Au-labeled TCP antibody, immunoreaction time, the pretreatment of sample pad and the preparation of stock solution of Au-TCP antibody that realize sensitivity, selectivity and direct detection of TCP. Under optimal conditions, this biosensor displays a highly linear range of 0.625-20 ng/mL TCP, with a detection limit of 0.47 ng/mL. Moreover, the immunosensor was successfully used for direct analysis of human saliva sample without any pretreatment. These results demonstrate that this Au nanoparticles-based immunochromatographic test strip (ITS) provides a simple, accurate, and quantitative tool for TCP detection and holds a great promise for point-of-care and in-field analysis of other biomarkers. [Description provided by NIOSH]
• Subjects:
  Antibodies Antigens Biochemistry Biomarkers Chromatography Colorimetry Energy Metabolism Environmental Monitoring Immune System Insecticides Metals Occupational Health Organophosphates Safety

  More +
• Keywords:
  Analytical-instruments Analytical-processes Antibody-response Au Nanoparticles Author Keywords: Trichloropyridinol Biochemical-tests Biological-monitoring Biomarker Chromatographic-analysis Detectors Equipment-reliability Exposure-assessment Immunochemistry Immunochromatographic Biosensor Immunological-tests Metabolites Metal-compounds Nanotechnology Organo-phosphorus-compounds Organo-phosphorus-pesticides Organophosphorus Pesticides Performance-capability Pesticides Reaction-rates Sample-preparation Testing-equipment

  More +
• ISSN:
  0039-9140
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  261-267
• Volume:
  114
• NIOSHTIC Number:
  nn:20043943
• Citation:
  Talanta 2013 Sep; 114:261-267
• Contact Point Address:
  Dan Du, Pacific Northwest National Laboratory, Richland, WA 99352, USA
• Email:
  dudan@mail.ccnu.edu.cn
• CAS Registry Number:
  3,5,6-Trichloro-2-pyridinol (CAS RN 6515-38-4) ; Chlorpyrifos (CAS RN 2921-88-2) ; Gold (CAS RN 7440-57-5)
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Battelle Pacific Northwest Laboratories
• Peer Reviewed:
  True
• Start Date:
  20060901
• Source Full Name:
  Talanta
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:234924ce0587b86c1046a3f274d708357abfd4f7f753e2d6511b6eac0c3aa3eb9ea38f97731b94261a072c313fd616ef348c5102c23d8df7f602f6a21df2a6ce
• Download URL:
  https://stacks.cdc.gov/view/cdc/199809/cdc_199809_DS1.pdf
• File Type:
  [PDF - 2.63 MB ]