Quantum dot-based immunochromatographic fluorescent biosensor for biomonitoring trichloropyridinol, a biomarker of exposure to chlorpyrifos

Details

• Personal Author:
  Du D ; Li Y ; Lin Y ; Smith JN ; Timchalk C ; Wang J ; Zou Z
• Description:
  A novel and portable fluorescent sensor that integrates an immunochromatographic test strip assay (ITSA) with a quantum dot (QD) label and a test strip reader was described in this study for simple, rapid, and sensitive biomonitoring of an organophosphorus pesticide metabolite. The principle of this sensor is based on a competitive immunoreaction that was performed on an immunochromatographic test strip, where analytes compete with competitors (QD-conjugated analogs) to bind to antibodies on a test zone. Captured QDs serve as signal vehicles for fluorescent readout. In this work, 3,5,6-trichloropyridinol (TCP) is used as a model analyte to demonstrate the performance of the immunosensor. QD-TCP conjugates were synthesized and characterized with X-ray photoelectron spectroscopy (XPS) and fluorescence spectroscopy. Some parameters (e.g., the amount of QD-modified TCP and immunoreaction time) that govern sensitivity and reproducibility of ITSA were optimized. Under optimal conditions, the sensor has a wide dynamic range and is capable of detecting a minimum 1.0 ng/mL TCP standard analyte in 15 min. The sensor has been successfully applied for detection of TCP spiked in rat plasma with average recovery of 102.0%. Results demonstrate that this sensor provides a rapid, clinically accurate, and quantitative tool for TCP detection and shows great promise for in-field and point-of-care (POC) quantitative testing and screening for metabolite biomarkers, e.g., TCP, for humans exposed to pesticides. [Description provided by NIOSH]
• Subjects:
  Biological Assay Biomarkers Chromatography Energy Metabolism Immune System Insecticides Occupational Health Organophosphates Safety
• Keywords:
  Bioassays Biological-monitoring Chromatographic-analysis Detectors Fluorescence-spectrometry Immune-reaction Immunochemistry Immunological-tests Metabolites Organo-phosphorus-pesticides Pesticides Testing-equipment

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• ISSN:
  0003-2700
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  82
• Issue:
  12
• NIOSHTIC Number:
  nn:20037706
• Citation:
  Anal Chem 2010 Jun; 82(12):5125-5133
• Contact Point Address:
  Jun Wang, Pacific Northwest National Laboratory, Richland, WA 99352
• Email:
  jun.wang@pnl.gov
• CAS Registry Number:
  3,5,6-Trichloro-2-pyridinol (CAS RN 6515-38-4) ; Chlorpyrifos (CAS RN 2921-88-2)
• Federal Fiscal Year:
  2010
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Battelle Pacific Northwest Laboratories
• Peer Reviewed:
  True
• Start Date:
  20060901
• Source Full Name:
  Analytical Chemistry
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8bbb247a4f63e52a62bc0c14accd677b8e27f490d4679140c46f4663120df299388109bd1d31f6cfbb0f723ba8f216798d0fbe93eeb33fa3757ce831086ebb87
• Download URL:
  https://stacks.cdc.gov/view/cdc/191699/cdc_191699_DS1.pdf
• File Type:
  [PDF - 2.53 MB ]