A novel immunochromatographic electrochemical biosensor for highly sensitive and selective detection of trichloropyridinol, a biomarker of exposure to chlorpyrifos

Details

• Personal Author:
  Du D ; Lin Y ; Liu F ; Lu D ; Smith JN ; Timchalk C ; Wang H ; Wang J ; Wang L ; Zou Z
• Description:
  We present a novel portable immunochromatographic electrochemical biosensor (IEB) for simple, rapid, and sensitive biomonitoring of trichloropyridinol (TCP), a metabolite biomarker of exposure to organophosphorus insecticides. Our new approach takes the advantage of immunochromatographic test strip for a rapid competitive immunoreaction and a disposable screen-printed carbon electrode for a rapid and sensitive electrochemical analysis of captured HRP labeling. Several key experimental parameters (e.g. immunoreaction time, the amount of HRP labeled TCP, concentration of the substrate for electrochemical measurements, and the blocking agents for the nitrocellulose membrane) were optimized to achieve a high sensitivity, selectivity and stability. Under optimal conditions, the IEB has demonstrated a wide linear range (0.1-100 ng/ml) with a detection limit as low as 0.1 ng/ml TCP. Furthermore, the IEB has been successfully applied for biomonitoring of TCP in the rat plasma samples with in vivo exposure to organophosphorus insecticides like Chlorpyrifos-oxon (CPF-oxon). The IEB thus opens up new pathways for designing a simple, rapid, clinically accurate, and quantitative tool for TCP detection, as well as holds a great promise for in-field screening of metabolite biomarkers, e.g., TCP, for humans exposed to organophosphorus insecticides. [Description provided by NIOSH]
• Subjects:
  Antibodies Biomarkers Chromatography Energy Metabolism Environmental Monitoring Enzymes Immune System Insecticides Occupational Health Organophosphates Safety

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• Keywords:
  Acetylcholinesterase Antibody-response Author Keywords: Immunochromatographic Electrochemical Biosensor Biological-monitoring Biomarker Chromatographic-analysis Competitive Immunoreaction Electrochemical-analysis Enzyme-activity Enzyme-complexes Exposure-assessment Immune-reaction Immunochemistry In-vivo-study Metabolites Organo-phosphorus-compounds Organo-phosphorus-pesticides Organophosphorus Insecticides Peroxidases Sensitivity-testing Trichloropyridinol

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• ISSN:
  0956-5663
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  26
• Issue:
  6
• NIOSHTIC Number:
  nn:20038748
• Citation:
  Biosens Bioelectron 2011 Feb; 26(6):2835-2840
• CAS Registry Number:
  3,5,6-Trichloro-2-pyridinol (CAS RN 6515-38-4) ; Chlorpyrifos (CAS RN 2921-88-2) ; Chlorpyrifos oxon (CAS RN 5598-15-2) ; Chlorpyrifos-methyl (CAS RN 5598-13-0)
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Battelle Pacific Northwest Laboratories
• Peer Reviewed:
  True
• Start Date:
  20060901
• Source Full Name:
  Biosensors and Bioelectronics
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:729811fe55e974d03a93ff0df07133d549c13450102821cb8012d37a765be0ec72dc3c5ac74e3492c8d36fe97f03386961a34c4b9c91b426259f83f6699f3eb7
• Download URL:
  https://stacks.cdc.gov/view/cdc/192355/cdc_192355_DS1.pdf
• File Type:
  [PDF - 715.95 KB ]