Exposure to Stainless Steel Welding Fume Suppresses Lung Defense Function After Infection in Rats

Details

• Personal Author:
  Antonini JM ; Roberts, Jennifer R. ; Taylor MD
• Description:
  Pulmonary infections are increased in severity, duration, and frequency among full-time welders. The objective was to examine the mechanisms by which a stainless steel welding fume may alter lung defense against infection. At day 0, male Sprague-Dawley rats were intratracheally instilled with a manual metal arc, stainless steel welding fume (MMA-SS) at a concentration of 1.0 mg/100 g body weight or saline (vehicle control). At day 3, the MMA-SS- and saline-treated rats were intratracheally inoculated with 5,000 Listeria monocytogenes. At days 6, 8 and 10, left lungs were removed, homogenized, cultured overnight, and colony-forming units counted to assess pulmonary bacterial clearance. Right lungs were lavaged to recover phagocytes and lavage fluid to measure the production of the anti-microbial agent, nitric oxide (NO) and immuno-modulatory cytokines, including IL-2 and IL-10. Exposure to the fume before infection significantly slowed the pulmonary clearance of the bacteria and severely damaged the lungs compared to control. Phagocytic cell NO production and lavage fluid IL-2 were significantly reduced in the MMA-SS + L. monocytogenes group early after infection compared to the saline + L. monocytogenes group. Pretreatment with MMA-SS before infection caused a significant increase in lavage fluid IL-10. In conclusion, the MMA-SS-induced suppression of lung defense against infection in rats involves a reduction in cellular production of NO and IL-2 as well as an increase in anti-inflammatory IL-10 production. [Description provided by NIOSH]
• Subjects:
  Animals Laboratories Lung Lung Diseases Metal Workers Occupational Health Respiratory System Respiratory Tract Diseases Safety Welding
• Keywords:
  Animal-studies Laboratory-animals Lung-disease Lung-disorders Lung-irritants Pulmonary-system-disorders Stainless-steel Steel-industry Steelworkers Welders Welders-lung

  More +
• ISSN:
  1073-449X
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  167
• Issue:
  7
• NIOSHTIC Number:
  nn:20023042
• Citation:
  Am J Respir Crit Care Med 2003 Apr; 167(7):A258
• Email:
  jga6@cdc.gov
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  2003
• NORA Priority Area:
  Work Environment and Workforce: Mixed Exposures
• Peer Reviewed:
  False
• Source Full Name:
  American Journal of Respiratory and Critical Care Medicine, 2003 International Conference, The American Thoracic Society, Seattle, WA, May 16-21, 2003
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:09421dd72235eba853f62464829a0af33cd30ccd43452785cc40458411223f2b43e3ed35859b94a6a43eea0ca887da360d880ddc05e2f101297f4530cac1dc95
• Download URL:
  https://stacks.cdc.gov/view/cdc/199628/cdc_199628_DS1.pdf
• File Type:
  [PDF - 144.31 KB ]