Intratracheal Instillation of Welding Fumes Alters the Pulmonary Clearance of Listeria monocytogenes in the Rat

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• Personal Author:
  Antonini JM ; Charron TG
• Description:
  Epidemiological studies have shown an increased incidence of respiratory illness in welders. Stainless steel (SS) welding fumes have been shown to have a greater effect on alveolar macrophage (AM) function than mild steel (MS) fumes. The objective of this study was to evaluate the effects of different welding fumes on the clearance of a bacterial pathogen from the lungs. Fumes were collected during flux-cored manual metal arc (MMA) and gas metal arc (GMA) welding using either S8 or MS consumable electrodes. The fume composition was: 1) GMA.sS: 52.3% Fe, 22.2% Cr, 18.3% Mn, 4.9% Ni, 2.3% Si; 2) GMA-MS: 89.2% Fe, 8.2% Mn, 2.6% Si; 3) MMA-SS.: 22.3% K, 19.4% Fe, 13.1 % Cr, 12.6% Si, 8.2% Ca, 8.0% Mn, 17.4% other. CDNAF rats were dosed intratracheally with saline (control) or the welding samples at a dose of J.O mg/100 g b wt. At 1 and 35 days postinstillation, 5000 Listeria monocytogenes were intratracheally instilled into the treated animals. Five days after intratracheal exposure to L. monocytogenes, the lungs and spleen were removed, homogenized, and cultured quantitatively on Brain Heart Infusion agar at 370 C. Colony forming units (CFUs) were counted after an overnight incubation. After a 1 day fume treatment, all three we1ding samples caused a significant decrease (p
• Subjects:
  Animals Chemistry Chemistry, Analytic Irritants Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety Welding

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• Keywords:
  Analytical-chemistry Analytical-methods Animal-studies Chemical-analysis Laboratory-animals Pulmonary-disorders Pulmonary-system-disorders Respiratory-irritants Respiratory-system-disorders Stainless-steel Welders Welders-lung Welding-industry

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  48
• NIOSHTIC Number:
  nn:20027838
• Citation:
  Toxicologist 1999 Mar; 48(1-S):131
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 38th Annual Meeting, March 14-18, 1999, New Orleans, Louisiana
• Supplement:
  1-S
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:eeef56195957136854f9c71ed43f59a612d42b71f8310f34b781e40a65e56fefe0b3502b76e980aedf393f2802f0c1ec643ba863d6da2a76f23d98d69e06ede6
• Download URL:
  https://stacks.cdc.gov/view/cdc/197681/cdc_197681_DS1.pdf
• File Type:
  [PDF - 143.71 KB ]