Kinetics of Changes in Lymphocyte Sub-Populations in Mouse Lungs After Intrapulmonary Infection with M. bovis (Bacillus Calmette-Guerin) and Identity of Cells Responsible for IFNgamma Responses

Details

• Personal Author:
  Lewis, Daniel M. ; Saxena QB ; Saxena RK ; Simpson J ; Weissman, David N.
• Description:
  Gamma interferon (IFN) plays a key role in host defense against pulmonary mycobacterial infections. A variety of lymphocyte subsets may participate in producing pulmonary IFN responses, but their relative contributions after mycobacterial infection have not been clearly elucidated. To address this question, C57Bl/6 female mice were infected by intrapulmonary instillation of 2·5 104 BCG (Mycobacterium bovis Bacillus Calmette-Guerin). Lymphocyte populations in lung interstitium were examined at different time points after the infection. BCG load in lungs peaked between 4 and 6 weeks post-infection and declined to very low levels by the 12th week of infection. Recovery of lung interstitial lymphocytes doubled by 4-6 weeks after infection and declined thereafter. Flow cytometric analysis of the lung-derived lymphocytes revealed that about 5% of the these cells made IFN in control mice, and this baseline IFN production involved T (CD3+NK1.1), NK (CD3NK1.1+) and NKT (CD3+NK1.1+) cells. As the BCG lung infection peaked, the total number of CD3+ T cells in the lungs increased threefold at 5-6 weeks post-infection. There was a marked increase (sixfold) in the number of T cells secreting IFN 5-6 weeks post-infection. Some increase was also noted in the NKT cells making IFN, but the numbers of NK cells making IFN in BCG-infected lungs remained unaltered. Our results suggest that whereas NK and NKT cells contribute to baseline IFN secretion in control lungs, expansion in the IFN-producing T-cell population was essentially responsible for the augmented response seen in lungs of BCG-infected mice. [Description provided by NIOSH]
• Subjects:
  Animals Blood Cells, Cultured Laboratories Lung Lung Diseases Microbiology Occupational Health Respiratory System Respiratory Tract Diseases Safety

  More +
• Keywords:
  Animal-studies Author Keywords: Interferon-gamma BCG Infection Cell-cultures Cell-morphology Host Laboratory-animals Lymphocytes Mice Microorganisms Pulmonary-disorders Pulmonary-function Resistance Models T Cells

  More +
• ISSN:
  0954-7894
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  405-410
• Volume:
  128
• Issue:
  3
• NIOSHTIC Number:
  nn:20022967
• Citation:
  Clin Exp Allergy 2002 Jun; 128(3):405-410
• Contact Point Address:
  Dr; Daniel M. Lewis, Analytical Services Branch, HELD, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505, USA
• Email:
  dml1@cdc.gov
• Federal Fiscal Year:
  2002
• NORA Priority Area:
  Disease and Injury: Infectious Diseases
• Peer Reviewed:
  True
• Source Full Name:
  Clinical and Experimental Allergy
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d6f02ab019929d5c7c0c0e3af3a4f5f569057ab174d519d72eae30293deee4b0bee54046d4cd45752cb496cf408b7a52e7c3384c6a7e565d8bc92e94348ea8c9
• Download URL:
  https://stacks.cdc.gov/view/cdc/199569/cdc_199569_DS1.pdf
• File Type:
  [PDF - 97.07 KB ]