Influence of Hyperthyroidism on Rat Lung Cytokine Production and Nuclear Factor-kappaB Activation Following Ozone Exposure

Details

• Personal Author:
  Brumbaugh K ; Ding M ; Huffman LJ ; Prugh DJ
• Description:
  Results from previous studies indicate that hyperthyroidism increases the risk of ozone-induced lung toxicity. To better understand the processes that might contribute to the increased pulmonary inflammatory response to ozone in hyperthyroidism, we evaluated bronchoalveolar lavage fluid levels of selected cytokines in control and hyperthyroid rats after exposure to air or ozone. In addition, we assessed whether there is a relative increase in nuclear factor-kappa B (NF-kappaB) binding activity in cells harvested by bronchoalveolar lavage from hyperthyroid rats following the inhalation of ozone. A hyperthyroid condition was induced by the administration of thyroxine (0.5 mg/kg body weight) for 7 days. Control rats received vehicle injections. The animals were then exposed by inhalation to air or ozone (2 ppm for 3 h) and studied 18 h following the exposure. Bronchoalveolar lavage levels of MIP-2 and MCP-1 were increased in both control and hyperthyroid rats by ozone exposure. However, the increases in hyperthyroid rats were much greater, MIP-2 1.5-fold and MCP-1 11-fold, when compared to levels in controls following ozone. These changes appeared to be relatively specific; bronchoalveolar lavage fluid levels of interleukin (IL)-6, IL-4, and IL-10 were generally low or nondetectable across all of the studied groups at the 18-h postexposure time point. We also found that NF-kappaB binding activity was increased at both 4 and 18 h following ozone exposure in bronchoalveolar lavage cell extracts from hyperthyroid rats relative to the activity in control samples. Collectively, these results suggest that mechanisms contributing to the enhanced pulmonary inflammatory response to ozone in a hyperthyroid state include an increase in NF-kappaB activation and an upregulation of chemokine production. [Description provided by NIOSH]
• Subjects:
  Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Bronchial-cancer In-vitro-studies Inhalation-studies Lung-disease Pulmonary-system-disorders Respiratory-system-disorders Thyroid-gland-disorders
• ISSN:
  0895-8378
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  14
• Issue:
  11
• NIOSHTIC Number:
  nn:20022932
• Citation:
  Inhal Toxicol 2002 Nov; 14(11):1161-1174
• Contact Point Address:
  Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, M/S 2015, Morgantown, West Virginia 26505
• Federal Fiscal Year:
  2003
• NORA Priority Area:
  Disease and Injury: Fertility and Pregnancy Abnormalities
• Peer Reviewed:
  True
• Source Full Name:
  Inhalation Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0dc4ab218d2b720271f68919a8ed14ceb448e588257e83b1845e7987d5b3cd9ae023d04dc21c700dbd8333d14d1eb29891b3b945409ce2575ff971abdeeb18df
• Download URL:
  https://stacks.cdc.gov/view/cdc/199541/cdc_199541_DS1.pdf
• File Type:
  [PDF - 374.57 KB ]