Chronic Smoking Enhances Tachykinin Synthesis and Airway Responsiveness in Guinea Pigs

Details

• Personal Author:
  Kashon ML ; Krajnak, Kristine M. ; Kwong, Kyei-Sing ; Lee LY ; Wise PM ; Wu ZX
• Description:
  This study tests the hypothesis that the bronchial hyperreactivity induced by chronic cigarette smoke (CS) exposure involves the increased expression and release of tachykinins and calcitonin gene-related peptide (CGRP) from afferent nerve fibers innervating the airways. In guinea pigs chronically exposed to CS (20 min twice daily for 14-17 d), peak response in total lung resistance to capsaicin (1.68 microg/kg, intravenously) was significantly greater than that evoked by the same dose of capsaicin in control (air-exposed) animals. This augmented response in CS-exposed animals was abolished after treatment with CP-99994 and SR-48968, the neurokinin (NK)-1 and NK-2 receptor antagonists, suggesting the involvement of tachykinins in chronic CS-induced airway hyperresponsiveness (AHR). Further, substance P (SP)-like immunoreactivity (LI) and CGRP-LI in the airway tissue were significantly greater in the CS animals than in the control animals. Finally, beta-preprotachykinin (PPT, a splice variant from the PPT A gene encoding tachykinins including SP and NKA) messenger RNA levels as measured by in situ hybridization histochemistry displayed a significant increase in jugular ganglion neurons but not in dorsal root or nodose ganglion neurons. These data suggest that chronic CS-induced AHR is related to an increase in SP synthesis and release in jugular ganglion neurons innervating the lungs and airways. [Description provided by NIOSH]
• Subjects:
  Animals Laboratories Lung Lung Diseases Occupational Exposure Occupational Health Respiratory System Respiratory Tract Diseases Safety Smokers Tobacco Products

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• Keywords:
  Airway-obstruction Airway-resistance Animal-studies Bronchial-cancer Chronic-exposure Cigarette-smoking Exposure-levels Laboratory-animals Lung-disease Lung-disorders Lung-irritants Pulmonary-system-disorders Respiratory-system-disorders Smoking

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• ISSN:
  1044-1549
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Kentucky ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  299-305
• Volume:
  25
• Issue:
  3
• NIOSHTIC Number:
  nn:20021783
• Citation:
  Am J Respir Cell Mol Biol 2001 Sep; 25(3):299-305
• Contact Point Address:
  Lu-Yuan Lee, Ph.D., Dept. of Physiology, MS511A Chandler Medical Center, 800 Rose St., University of Kentucky, Lexington, KY 40536
• Email:
  lylee@pop.uky.edu
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  American Journal Respiratory Cell and Molecular Biology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:262c110cca1acd35995b905cb219671e59a28992a0242793c713271945cfc3e8e26f2be50d979d8f6e49541cbec06dbb57dc3301312bad21d48de6b548b92214
• Download URL:
  https://stacks.cdc.gov/view/cdc/198890/cdc_198890_DS1.pdf
• File Type:
  [PDF - 167.14 KB ]