Immunoglobulin Responses to Experimental Silicosis

Details

• Personal Author:
  Banks DE ; Huang SH ; Hubbs, Ann F. ; Ma JKH ; Rojanasakul Y ; Schnabel PC ; Stanley CF ; Vallyathan V ; Weissman, David N.
• Description:
  Silicosis is a crippling fibrotic lung disease induced by inhalation of crystalline silica. One feature of silicosis is systemic and pulmonary immune dysfunction characterized in part by elevations in serum and bronchoalveolar lavage (BAL) immunoglobulins. A major specific aim of the current report was to demonstrate that an experimental model of silicosis previously well characterized for the development of pulmonary inflammation and fibrosis would also exhibit increased levels of serum and BAL IgG and IgM similar to those of human silicosis. We also sought to document the anatomic compartments responsible for these immunoglobulin responses. To address these specific aims, we compared levels of IgG and IgM in serum and BAL from rats with experimental silicosis induced by inhalation of silica with levels of these immunoglobulins in titanium dioxide (TiO(2))- and sham (air)-exposed controls. The ability of mononuclear cell populations from lung, lung-associated lymph node, and spleen to produce IgG and IgM ex vivo were also compared. We found that experimental silicosis was associated with elevated IgG and IgM levels in blood and BAL relative to the control groups. Our findings also suggested that draining lung-associated lymph nodes (LALN) were the most important sites for increased IgG and IgM production in experimental silicosis, with lungs contributing to a lesser degree. Increased production in the LALN appeared related to marked expansion in total numbers, but not relative proportion, of B lymphocytes. [Description provided by NIOSH]
• Subjects:
  Blood Immune System Lung Occupational Health Respiratory System Safety Silicosis
• Keywords:
  Author Keywords: Silicosis Bronchoalveolar Lavage IgG IgM Immune-system Immunoglobulins Lymphocyte Lymphocytes Pulmonary-system Quartz Rat Titanium Dioxide

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  108-117
• Volume:
  59
• Issue:
  1
• NIOSHTIC Number:
  nn:20021758
• Citation:
  Toxicol Sci 2001 Jan; 59(1):108-117
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  Toxicological Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:edf2c5ededc275b14b81c3c8554b028c32f2e08612551f350282bbed696790215a183d9c4d4b0949543012a4b5a3af3d94b0448c9567e9497e4abd40f44bb6ca
• Download URL:
  https://stacks.cdc.gov/view/cdc/198867/cdc_198867_DS1.pdf
• File Type:
  [PDF - 368.60 KB ]