Interleukin 1-Dependent Regulation of Interleukin 6 Production by Wounding in Human Epithelial Keratinocyte

Details

• Personal Author:
  Gallucci RM ; Luster MI ; Simeonova PP ; Sugawara T
• Description:
  Wound healing in the skin is accompanied by inflammation. Increasing evidence indicates that the proinflammatory cytokine interleukin 6 (IL-6), plays an important role in wound healing. However, the mechanisms of IL-6 production and involvement in wound healing remain unclear. The present study was designed to examine the molecular mechanism of IL-6 production, and its involvement in wound healing in normal human epidermal keratinocyre (NHEK) using an in vitro wounding model. Herein, we demonstrate that IL-6 mRNA expression and immunoreactive IL-6 release were increased following wounding. The transcription factors NF-KB and NF-IL6 (C/EBPB), which are known to be involved in IL-6 induction, were also activated following wounding. Addition of IL-1 increased IL-6 production and induced NF-KB and C/EBPB activation in NHEK. Exogenous IL-1 receptor antagonist inhibited IL-6 mRNA expression and transcription factor activation following wounding. Immunoreactive IL-1 was detected in the culture medium following wounding, but mRNA was not increased. Additionally, mRNA expression of keratins 1 and 10, which are differentiation markers of keratinocyres, was decreased in wounded NHEK or keratinocytes treated with exogenous IL-6. Thus, these data indicate that the primary stimulus for IL-6 production in wounded epidermis is aurocrine/paracrine stimulus with IL-l, and the mechanism by which IL-6 aids in wound healing is associated with the inhibition of differentiation in epidermal keratinocytes. [Description provided by NIOSH]
• Subjects:
  Occupational Health Safety Skin Skin Diseases
• Keywords:
  Cell-biology Cellular-reactions Exposure-levels In-vitro-studies Skin-disorders
• ISSN:
  1096-6080
• Publisher:
  Oxford University Press
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  60
• Issue:
  1
• NIOSHTIC Number:
  nn:20021460
• Citation:
  Toxicologist 2001 Mar; 60(1):176
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 40th Annual Meeting, March 25-29, 2001, San Francisco, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:62b3fee1271f85f2343f2c161dd51e9176632a8588217fac609d839623abb3d3ccb5fbf5fdb1aa6ac38164bc00beb7ad5a61b372942d681bd464a22bbbfb2325
• Download URL:
  https://stacks.cdc.gov/view/cdc/198768/cdc_198768_DS1.pdf
• File Type:
  [PDF - 125.85 KB ]