An In Vivo Animal Model for the Investigation of Acute and Chronic Skeletal Muscle Injury

Details

• Personal Author:
  Cutlip, Robert G. ; Geronilla K ; Miller R ; Mowrey K ; Wirth, Oliver
• Description:
  An in vivo model to study skeletal muscle injury is described. A computer-controlled custom-designed rat dynamometer is used to control biomechanical inputs such as range of motion, velocity, acceleration, and number of repetitions to study skeletal muscle injury in rats. Anesthetized rats are placed supine in the dynamometer and the left foot placed in a load cell with the ankle axis aligned with the axis of rotation of the motor. Platinum electrodes are placed subcutaneously to branch either the peroneal nerve (to activate the dorsi flexor muscles of the hind limb) or the tibial nerve (to activate the plantar flexor muscles of the hind limb). The free ends of the wire electrodes are connected to a computer-controlled nerve stimulator. The dynamometer can be programmed to produced controlled angular movement about the ankle axis to generate isometric, concentric, and reciprocal concentric/eccentric muscle actions of either the plantar flexor or dorsi flexor muscles. This model has distinct advantages as compared to invasive in vitro or in situ preparations of isolated muscles or muscle fibers. Muscle response and injury can be studied in a more physiologically representative fashion with the neural and vascular supplies and muscle-tendon complexes intact. The non-invasive features of this model are well suited for the study of chronic muscle injury. When the biomechanical data from the dynamometer are combined with histological and biochemical analyses of muscle tissue, this model can provide comprehensive data for studying acute and chronic skeletal muscle pathomechanics. [Description provided by NIOSH]
• Subjects:
  Animals Laboratories Musculoskeletal Diseases Musculoskeletal System Occupational Health Safety
• Keywords:
  Animal Studies Injuries In Vivo Studies Laboratory Animals Models Muscle Tissue Musculoskeletal System Disorders Skeletal System Disorders
• ISBN:
  9781586031749
• ISSN:
  1384-2269
• Publisher:
  IOS Press
• Document Type:
  Text
• Genre:
  Proceedings
• Place as Subject:
  District of Columbia ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  394-401
• Volume:
  4
• NIOSHTIC Number:
  nn:20021283
• Citation:
  Advances in Occupational Ergonomics and Safety: XVth Annual Conference of the International Society for Occupational Ergonomics and Safety, June 4 - 7, 2001, Fairfax, Virgina. Bittner AC Jr., Champney PC, Morrissey SJ, eds. Washington, DC: IOS Press, 2001 Jan; 4:394-401
• Editor(s):
  Bittner AC Jr.; Champney PC; Morrissey SJ
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  False
• Source Full Name:
  Advances in Occupational Ergonomics and Safety: XVth Annual Conference of the International Society for Occupational Ergonomics and Safety, June 4 - 7, 2001, Fairfax, Virgina
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8713942f96bced9f47bb02f1a0eddc0cf98c3a265868407aa2bcd807de783d0faac5dd0be67fd1ace2e209081c1298ce66bb4f820f98678e0d44548c2c71a57c
• Download URL:
  https://stacks.cdc.gov/view/cdc/198673/cdc_198673_DS1.pdf
• File Type:
  [PDF - 354.61 KB ]