Ethanol-Mediated CYP1A1/2 Induction in Rat Skeletal Muscle Tissue

Details

• Personal Author:
  Gannett PM ; Harsh V ; Hobbs G ; Miller MR ; Riggs JE ; Smith C ; Stamm SC ; Stauber W
• Description:
  The causes of non-trauma-mediated rhabdomyolysis are not well understood. It has been speculated that ethanol-associated rhabdomyolysis may be attributed to ethanol induction of skeletal muscle cytochrome P450(s), causing drugs such as acetaminophen or cocaine to be metabolized to myotoxic compounds. To examine this possibility, the hypothesis that feeding ethanol induces cytochrome P450 in skeletal muscle was tested. To this end, rats were fed an ethanol-containing diet and skeletal muscle tissue was assessed for induction of CYP2E1 and CYP1A1/2 by immunohistochemical procedures; liver was examined as a positive control tissue. Enzymatic assays and Western blot analyses were also performed on these tissues. In one feeding system, ethanol-containing diets induced CYP1A1/2 in soleus, plantaris, and diaphragm muscles, with immunohistochemical staining predominantly localized to capillaries surrounding myofibers. Antibodies to CYP2E1 did not react with skeletal muscle tissue from animals receiving a control or ethanol-containing diet. However, neither skeletal muscle CYP1A1/2 nor CYP2E1 was induced when ethanol diets were administered by a different feeding system. Ethanol consumption can induce some cytochrome P450 isoforms in skeletal muscle tissue; however, the mechanism of CYP induction is apparently complex and appears to involve factors in addition to ethanol, per se. [Description provided by NIOSH]
• Subjects:
  Animals Enzymes Ethanol Laboratories Musculoskeletal Diseases Musculoskeletal System Occupational Health Safety
• Keywords:
  Animal Studies Ethanols Laboratory Animals Muscle Tissue Musculoskeletal System Disorders Skeletal System Skeletal System Disorders
• ISSN:
  0014-4800
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  223-232
• Volume:
  69
• Issue:
  3
• NIOSHTIC Number:
  nn:20029513
• Citation:
  Exp Mol Pathol 2000 Dec; 69(3):223-232
• Contact Point Address:
  Department of Biochemistry, P.O. Box 9142, West Virginia University Health Sciences Center, Morgantown, WV 26506-9142
• Email:
  mmiller@hsc.wvu.edu
• CAS Registry Number:
  Ethanol (CAS RN 64-17-5)
• Federal Fiscal Year:
  2001
• NORA Priority Area:
  Disease and Injury: Musculoskeletal Disorders of the Upper Extremities
• Performing Organization:
  West Virginia University, Morgantown, West Virginia
• Peer Reviewed:
  True
• Start Date:
  19920101
• Source Full Name:
  Experimental and Molecular Pathology
• End Date:
  20050531
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5cef52a786f8386a43adf18d2df4e6067e26a602f8f15d6de25ec9bf73bcc8aa5e210503457cf673a2443a7459fd5d6a9d8d2844d8bd3428fd0123cf7a95f071
• Download URL:
  https://stacks.cdc.gov/view/cdc/190470/cdc_190470_DS1.pdf
• File Type:
  [PDF - 209.04 KB ]