Alterations of Cancer-Related Genes in Tumor Cell Lines Derived from Cadmium-Transformed BALB/C-3T3 Cells

Details

• Personal Author:
  Joseph P ; Muchnok TK ; Ong T ; Whong, Wen-Zong ; Zhou G
• Description:
  The International Agency for Research on Cancer (IARC) has classified cadmium as a human carcinogen. A large number at workers are potentially exposed to this metal in various occupational settings. Considering the lack at information regarding the molecular mechanism(s) at cadmium carcinogenesis, studies were conducted to investigate the changes in copy number and expression at various cancer-related genes as a possible mechanism for carcinogenesis due to exposure to cadmium. BALB/c-3T3 cells were morphologically transformed with cadmium and the transformed cells were subcutaneously injected into nude mice to develop tumors. Cell lines established from these tumors were used in the present study. Differential PCR and RT-PCR were employed to determine changes in copy number and expression at p53, p16, K-ras, c-myc, c-fos, c-jun, c-sis and erbB1. Tumor cell lines showed a decrease in gene copy number and expression of p16 and erbB1 compared to the control BALB/c-3T3 cells. Gene copy number and gene expression of c-fos and c-jun were significantly higher in the tumor cell lines compared to those of the control cells. In spite of a significant increase in gene copy number of K-ras in tumor cells, no change was observed in its expression. Neither the copy number nor the expression of c-myc exhibited any significant change in the tumor cell lines. These results indicate that the observed changes in gene copy number and gene expression of the cancer-related genes may be at least partly responsible for the cellular transformation induced by cadmium. [Description provided by NIOSH]
• Subjects:
  Animals Carcinogens Genetics Laboratories Metals Neoplasms Occupational Exposure Occupational Health Safety Workplace
• Keywords:
  Animal Studies Cadmium Compounds Cancer Carcinogenesis Carcinogenicity Cell Transformation Genes Laboratory Animals Occupational Exposure Tumors Work Environment Workers

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• ISSN:
  0893-6692
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  33
• Volume:
  35
• NIOSHTIC Number:
  nn:20020973
• Citation:
  Environ Mol Mutagen 2000 Jan; 35(Suppl 31):33
• CAS Registry Number:
  Cadmium (CAS RN 7440-43-9)
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  False
• Source Full Name:
  Environmental and Molecular Mutagenesis
• Supplement:
  31
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a224e2d4e905778085b5442af24a5f37e0c17128aea1d1b766a8754d917dea20dac2ce42b07a86a2352a1883c6630c119cd8b37395775fce0a9641ffa5067cba
• Download URL:
  https://stacks.cdc.gov/view/cdc/198579/cdc_198579_DS1.pdf
• File Type:
  [PDF - 197.31 KB ]