Association Between Tumors Developed from Beryllium-Transformed Cells and Changes in Cancer-Related Genes

Details

• Personal Author:
  Joseph P ; Klishis ML ; Muchnok TK ; Ong T ; Whong, Wen-Zong
• Description:
  Beryllium is used extensively in various industrial applications and many workers are potentially exposed to toxic levels of this metal. Even though laboratory studies have documented the carcinogenic potential of beryllium in experimental animals. The underlying molecular mechanisms are poorly understood. In order to elucidate the molecular mechanisms of beryllium-induced tumorigenesis, we have studied the various cancer-related genes that were altered as a result of cellular transformation induced by beryllium. BALB/c-3T3 cells, morphologically transformed with beryllium, were subcutaneously injected into nude mice to develop tumors. DNA and ANA isolated from cell lines derived from the tumors were used to determine the alterations of various cancer-related genes, such as p53, p16, K-ras, c-myc, c-fos. c-jun, c-sis and erbB1. Alterations of the copy number and expression of the genes were studied by differential PCA and ATPCA, respectively. Both the gene copy number and expressions of K-ras, c-fos and c-jun were higher in the tumor cells compared to those of the control Balb/c-3T3 cells. In contrast, p16 exhibited a decrease in both the gene copy number and expression in the tumor cells compared to those of the control cells. These results suggest that beryllium-induced tumorigenesis is associated with alterations of various cancer-related genes, and these alterations may play a role in beryllium-induced tumorigenesis. [Description provided by NIOSH]
• Subjects:
  Animals Beryllium Carcinogens Genetics Laboratories Metals Neoplasms Occupational Exposure Occupational Health Safety
• Keywords:
  Animal Studies Beryllium Compounds Cancer Cell Transformation Genes Laboratory Animals Laboratory Testing Occupational Exposure Toxic Effects Tumorigenesis Tumorigens Tumors Workers

  More +
• ISSN:
  0893-6692
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  33
• Volume:
  35
• NIOSHTIC Number:
  nn:20020972
• Citation:
  Environ Mol Mutagen 2000 Jan; 35(Suppl 31):33
• CAS Registry Number:
  Beryllium (CAS RN 7440-41-7)
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  False
• Source Full Name:
  Environmental and Molecular Mutagenesis
• Supplement:
  31
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a5b4836299d27fae98e3889cad682b1e70147a27005162abb4b9abc2b71b918b2ddf3bf01a2e46ccbb8a3764451bf8fef45add54958cb84dc3bebca2ee5b75e0
• Download URL:
  https://stacks.cdc.gov/view/cdc/198578/cdc_198578_DS1.pdf
• File Type:
  [PDF - 203.17 KB ]