The Effect of Restraint on the Development of Allergic Contact Dermatitis Is Dependent upon the Timing of the Restraint

Details

• Personal Author:
  Flint MS ; Tinkle SS
• Description:
  Previous studies have shown that restraint stress modifies the development of allergic contact dermatitis (ACD). We hypothesized that the timing of restraint with respect to the chemical application influences restraint-induced modulation of ACD. Male BALB/c and C57BL/6 mice were sensitized on the flank on days I and 2 with 0.5% di-nitrofluorobenzene (DNFB) and challenged on the ear on day 6 with 0.25% DNFB. Mice were restrained for 2 hours prior to chemical application on day I only, on days 1 and 2, or on day 6 only. We assessed serum corticosterone to verify activation of the hypothalamic-pituitary-adrenal axis and ear swelling as a measure of the inflammatory/immune response. Restraint increased serum corticosterone 10 16 fold in both strains of mice. DNFB stimulated significant ear swelling in non-restrained mice. The ear swelling response was suppressed in DNFB-treated mice restrained for 2 hours on day 1 only, but was unchanged in DNFB-treated mice restrained on days 1 and 2. In contrast, mice restrained only on day 6 displayed significantly enhanced ear swelling, approximately 20% higher than levels measured in DNFB-treated, non-restrained mice. Administration of the glucocorticoid receptor antagonist, RU486, had little effect on the suppressed ear swelling response but did block the day 6 restraint-induced increase. These data demonstrate that timing of restraint affects the type and degree of modulation of chemical-induced ear swelling and that corticosterone may be more important in the enhanced ear swelling response than in the suppression of ear swelling. [Description provided by NIOSH]
• Subjects:
  Animals Hypersensitivity Immune System Laboratories Occupational Health Safety Skin Skin Diseases
• Keywords:
  Allergic Dermatitis Animal Studies Contact Allergies Contact Dermatitis Immune Reaction Laboratory Animals
• ISSN:
  0022-202X
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  114
• Issue:
  4
• NIOSHTIC Number:
  nn:20020930
• Citation:
  J Invest Dermatol 2000 May; 114(4):188
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  False
• Source Full Name:
  Journal of Investigative Dermatology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d74ca8ca792c94dd1c36ffedce9aa64a37227c670c7628582ae99e7d689fe1297b2a6dd0c2f437f07bac603e72a8897985b463ddf2ae49d4146aa98db57c5bb3
• Download URL:
  https://stacks.cdc.gov/view/cdc/198557/cdc_198557_DS1.pdf
• File Type:
  [PDF - 102.21 KB ]