Antioxidant Properties of (-)-Epicatechin-3-Gallate and Its Inhibition of Cr(VI)-Induced DNA Damage and Cr(IV)- or TPA-Stimulated NF-kB Activation

Details

• Personal Author:
  Castranova, Vincent ; Ding M ; Dong Z ; Leonard, Stephen S. ; Rojanasakul Y ; Shi X ; Vallyathan V ; Ye J
• Description:
  Electron spin resonance (ESR) spin trapping was utilized to investigate the scavenging effects on hydroxyl radicals (OH) and superoxide radicals (O2-) by (-)-epigallocatechin-3-gallate (EGCG), one of the major anticancer compounds in tea. The spin trap used was 5,5-dimethyl-pyrroline N-oxide (DMPO). The Fenton reaction (Fe2+ + H2O2-->Fe3+ + OH + OH-) was used as a source of OH radicals. EGCG efficiently scavenges OH radicals with reaction rate of 4.62 x 10(11) M(-1)sec(-1), which is an order of magnitude higher than several well recognized antioxidants, such as ascorbate, glutathione and cysteine. It also scavenges O2- radicals as demonstrated by using xanthine and xanthine oxidase system as a source of O2- radicals. Through its antioxidant properties, EGCG exhibited a protective effect against DNA damage induced by Cr(VI). EGCG also inhibited activation of nuclear transcription factor NF-kappaB induced by Cr(IV) and 12-o-tetradecanoylphorbol-13-acetate (TPA). The present studies provide a mechanistic basis for the reported anticarcinogenic properties of EGCG and related tea products. [Description provided by NIOSH]
• Subjects:
  Antioxidants Free Radicals Neoplasms Occupational Health Safety
• Keywords:
  Antiooxidants Antioxidation Author Keywords: Oxygen Derived Free Radicals Cancer Epigallocatechin-3-gallate Free-radical-generation Free-radicals Hydroxyl-groups Tea
• ISSN:
  0300-8177
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Minnesota ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  125-132
• Volume:
  206
• Issue:
  1
• NIOSHTIC Number:
  nn:20020808
• Citation:
  Mol Cell Biochem 2000 Mar; 206(1-2):125-132
• CAS Registry Number:
  Glutathione (CAS RN 70-18-8)
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  True
• Source Full Name:
  Molecular and Cellular Biochemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:38aa1b98363d3d55fb956b59ab118f4f8cfc75a8106f4863a63d5d6379ab7be5b972e84eb47b3bfd407ccea2a529128b0f2a7e4fa511b337b4529bc50fdee5ad
• Download URL:
  https://stacks.cdc.gov/view/cdc/198473/cdc_198473_DS1.pdf
• File Type:
  [PDF - 320.19 KB ]