Transforming and Carcinogenic Potential of Cadmium Chloride in BALB/C-3T3 Cells

Details

• Personal Author:
  Ensell MX ; Hubbs, Ann F. ; Keshava N ; Ong T ; Zhou G
• Description:
  A large number of workers are potentially exposed to cadmium during mining and processing. Therefore, there is a concern regarding the potential carcinogenic hazards of cadmium to exposed workers. Studies have been performed to determine if cadmium chloride (CdCl(2)) can induce morphological cell transformation, DNA from CdCl(2)-induced transformed cells can transform other mammalian cells, and the transformed cells induced by CdCl(2) can form tumors in nude mice. BALB/c-3T3 cells were treated with different concentrations of CdCl(2) for 72 h. The frequency of transformed foci from each treatment was determined after cells were cultured for 4 to 5 weeks. DNAs from five CdCl(2)-induced transformed cell lines were isolated and gene transfection assay was performed using NIH-3T3 cells. Non-transformed BALB/c-3T3 cells and cells from 10 transformed cell lines induced by CdCl(2) were injected into both axillary regions of nude mice. Mice were screened once a week for the appearance and size of tumors. CdCl(2) caused a statistically significant, concentration-related increase in the transformation frequency. DNA from all five CdCl(2)-induced transformed cell lines tested was found to induce varying degrees of transfection-mediated transformation in NIH-3T3 cells. All 10 CdCl(2)-induced transformed cell lines formed fibrosarcomas in nude mice within 39 days of inoculation. Within this time period, no tumors were found in nude mice injected with non-transformed BALB/c-3T3 cells. These results indicate that CdCl(2) is capable of inducing morphological cell transformation and that the transformed cells induced by CdCl(2) are potentially tumorigenic. [Description provided by NIOSH]
• Subjects:
  Animals Carcinogens Laboratories Mining Neoplasms Occupational Exposure Occupational Health Safety Workplace
• Keywords:
  Animal Studies Author Keywords: Cell Transformation BALB/C-3T3 Cells Cadmium Chloride Cadmium Compounds Carcinogenesis Carcinogenicity Cell Transformation Gene Transfection Laboratory Animals Mine Workers Mining Industry Morphology Occupational Exposure Tumorigenicity Tumors Work Environment Worker Health

  More +
• ISSN:
  0027-5107
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  23-28
• Volume:
  448
• Issue:
  1
• NIOSHTIC Number:
  nn:20020721
• Citation:
  Mutat Res 2000 Mar; 448(1):23-28
• Contact Point Address:
  Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, m/s 3014, 1095 Willowdale Road, Morgantown, WV 26505-2845, USA
• Email:
  too2@cdc.gov
• CAS Registry Number:
  Cadmium (CAS RN 7440-43-9) ; Cadmium chloride (CAS RN 10108-64-2)
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  True
• Source Full Name:
  Mutation Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8e5a091ae9fc43f6c5a6f265a6b384222e752be37e6881955cf9ff4c9a9f01aa03a45a13782c382a483b59acb66e74a0d8d9ebeac88c90243fa882359a32cd99
• Download URL:
  https://stacks.cdc.gov/view/cdc/198396/cdc_198396_DS1.pdf
• File Type:
  [PDF - 252.01 KB ]