Development of a Combined Irritancy/Phenotypic Analysis Assay for the Identification and Differentiation of Chemicals with the Potential to Elicit Irritation, IgE-Mediated, or T Cell Mediated Hypersensitivity Responses

Details

• Personal Author:
  Manetz TS ; Meade BJ
• Description:
  Allergic and irritant dermatitis account for 15-20% of reported occupational diseases, resulting in an estimated annual loss of one billion dollars due to decreased productivity (NIOSH, 1996). There remains a need to develop rapid, reproducible, cost effective methods to screen chemicals for their ability to induce these responses. To this end several murine models have been developed. The irritancy assay, local lymph node assay, and serum total IgE-ELISA represent three models proposed to identify chemical irritants, chemical sensitizers and IgE-medicated hypersensitivity-inducing chemicals, respectively (Kimber et al., 1994; Potter et al., 1995; Woolhiser et al., 1998). Using flow cytometric analysis, Katona et al., (1983) observed an increased level of IgE+ draining mesenteric lymph node cells in mice undergoing an IgE response to parasitic infection with Nippostrongylus brasiliensis. Previous studies in this lab (data not shown) and others (Sikorski et al., 1996; Gerberick et al., 1997) have shown an elevation in the percent of B220+draining lymph node cells following dermal exposure to T-cell medicated hypersensitivity-inducing allergens. Using three end points: the percent increase in ear swelling, the percent increase in IgE+B220+draining lymph node cells, and the percent increase in B220+draining lymph node cells, these studies investigate the potential use of a single combined murine irritancy/phenotypic analysis assay to identify and differentiate chemicals with the capacity to elicit irritation, IgE-mediated, or T-cell medicated hypersensitivity responses. [Description provided by NIOSH]
• Subjects:
  Animals Chemistry Dermatitis Hypersensitivity Laboratories Occupational Diseases Occupational Health Safety Skin Skin Diseases Workplace

  More +
• Keywords:
  Allergens Allergic-dermatitis Animal-studies Author Keywords: Hypersensitivity Chemical-analysis Draining Lymph Node Flow Cytometry Irritation Laboratory-animals Methods Development Models Occupational-diseases Occupational Health And Safety Work-environment Work Environment

  More +
• ISSN:
  0271-3586
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  136-138
• Volume:
  36
• NIOSHTIC Number:
  nn:20000853
• Citation:
  Am J Ind Med 1999 Sep; 36(S1):136-138
• Contact Point Address:
  B. Jean Meade, National Institute for Occupational Safety and Health, 1095 Willowdale Rd MS 4020, Morgantown, WV 26505
• Email:
  bhm8@cdc.gov
• CAS Registry Number:
  Acetone (CAS RN 67-64-1) ; Dinitrofluorobenzene (CAS RN 70-34-8) ; Quaternary ammonium compounds, alkylbenzyldimethyl, chlorides (CAS RN 8001-54-5) ; Sodium dodecyl sulfate (CAS RN 151-21-3) ; Tetradecane (CAS RN 629-59-4) ; Toluene 2,4-diisocyanate (CAS RN 584-84-9)
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  True
• Source Full Name:
  American Journal of Industrial Medicine
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a357b394bc16fc43db5ac30fd09824d63a404bc4cdc811899f8a834074fb7015ed86e4052efe08ed76e23f5508a26810fe6aae70ca9384f3cf376243504dd5f2
• Download URL:
  https://stacks.cdc.gov/view/cdc/198222/cdc_198222_DS1.pdf
• File Type:
  [PDF - 79.02 KB ]