Scavenging of Superoxide Anion Radical by Chaparral

Details

• Personal Author:
  Cosma G ; Gardner H ; Shi X ; Starks K ; Vallyathan V ; Zang LY
• Description:
  Chaparral is considered to act as an antioxidant. However, the inhibitory effects of chaparral on specific radical species are not well understood. Using electron paramagnetic resonance (EPR) spectroscopy in combination with spin trapping techniques, we have found that chaparral scavenges superoxide anion radical (O2·-) in a dose-dependent manner. 5,5-dimethyl-lpyrroline-N-oxide (DMPO) was used as a spin trapping agent and the reaction of xanthine and xanthine oxidase as a source of O2·-. The kinetic parameters, IC50 and Vmax, for chaparral scavenging of O2·- were found to be 0.899 g/mL and 8.4 ng/mL/sec, respectively. The rate constant for chaparral scavenging O2·- was found to be 1.22 x 106 g-1s-1. Our studies suggest that the antioxidant properties of chaparral may involve a direct scavenging effect of the primary oxygen radical, O2·-. [Description provided by NIOSH]
• Subjects:
  Antioxidants Diet Free Radicals Occupational Health Safety
• Keywords:
  Antioxidation Dietary-effects Dose-response Free-radicals Health-care Plant-substances Plants Statistical-analysis
• ISSN:
  0300-8177
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; OSHA Region 3 ; OSHA Region 8 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  157-161
• Volume:
  196
• Issue:
  1
• NIOSHTIC Number:
  nn:20027817
• Citation:
  Mol Cell Biochem 1999 Jun; 196(1):157-161
• Contact Point Address:
  L.-Y. Zang, Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505-2888, USA
• CAS Registry Number:
  Oxygen (CAS RN 7782-44-7)
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  True
• Source Full Name:
  Molecular and Cellular Biochemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:69a439f20d5f55589feeaf3b9435a7ff7e4081d731ffc79c488940bfea9e69c2add858d2aee3a825234e40ac62782e9c3f950aea870f3c103e9f67500bb0e158
• Download URL:
  https://stacks.cdc.gov/view/cdc/197672/cdc_197672_DS1.pdf
• File Type:
  [PDF - 66.76 KB ]