Vanadate Induces Apoptosis in Epidermal JB6 P+ Cells via Hydrogen Peroxide-Mediated Reactions

Details

• Personal Author:
  Castranova, Vincent ; Ding M ; Leonard, Stephen S. ; Millecchia L ; Robinson VA ; Shi X ; Vallyathan V ; Ye J ; Zhang X
• Description:
  Apoptosis is a physiological mechanism for the control of DNA integrity in mammalian cells. Vanadium induces both DNA damage and apoptosis. It is suggested that vanadium-induced apoptosis serves to eliminate DNA-damaged cells. This study is designed to clarify a role of reactive oxygen species in the mechanism of apoptosis induced by vanadium. We established apoptosis model with murine epidermal JB6 P+ cells in the response to vanadium stimulation. Apoptosis was detected by a cell death ELISA assay and morphological analysis. The result shows that apoptosis induced by vanadate is dose-dependent, reaching its saturation level at a concentration of 100 M vanadate. Vanadyl (IV) can also induce apoptosis albeit with lesser potency. A role of reactive oxygen species was analyzed by multiple reagents including specific scavengers of different reactive oxygen species. The result shows that vanadate-induced apoptosis is enhanced by NADPH, superoxide dismutase and sodium formate, but was inhibited by catalase and deferoxamine. Cells exposed to vanadium consume more molecular oxygen and at the same time, produce more H2O2 as measured by the change in fluorescence of scopoletin in the presence of horseradish peroxidase. This change in oxygen consumption and H2O2 production is enhanced by NADPH. Taken together, these results show that vanadate induces apoptosis in epidermal cells and H2O2 induced by vanadate plays a major role in this process. [Description provided by NIOSH]
• Subjects:
  Carcinogens Chemistry Chemistry, Analytic DNA Epidemiology Free Radicals Hazardous Substances Irritants Lung Lung Diseases Neoplasms Occupational Health Peroxides Respiratory System Respiratory Tract Diseases Safety Skin Skin Diseases Vanadium Compounds

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• Keywords:
  Analytical-chemistry Analytical-methods Analytical-models Cancer Cell-morphology Chemical-analysis DNA-damage Free-radicals Lung-disorders Nuclear-reactions Pulmonary-disorders Pulmonary-system-disorders Respiratory-irritants Respiratory-system-disorders Skin-cancer Skin-diseases Skin-disorders Skin-exposure Skin-irritants Skin-lesions Skin-tumors Statistical-analysis Toxic-materials Toxins Tumors Vanadium-compounds

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• ISSN:
  0300-8177
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Indiana ; Maryland ; Missouri ; OSHA Region 3 ; OSHA Region 5 ; OSHA Region 7 ; Pennsylvania ; West Virginia ; Wisconsin
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  9-17
• Volume:
  202
• Issue:
  1
• NIOSHTIC Number:
  nn:20027816
• Citation:
  Mol Cell Biochem 1999 Dec; 202(1-2):9-17
• CAS Registry Number:
  Hydrogen peroxide (CAS RN 7722-84-1) ; Vanadium (CAS RN 7440-62-2)
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  True
• Source Full Name:
  Molecular and Cellular Biochemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ee8cb9e02c5c5366a658856133aff5073631174fcd841e27afaf4d80ea0a52dfac3022fb76c9675837a06599f9e9bd2b926e930abcded07b9818658fb3bc3a6d
• Download URL:
  https://stacks.cdc.gov/view/cdc/197671/cdc_197671_DS1.pdf
• File Type:
  [PDF - 203.11 KB ]