In Vivo Detection of Vitamin E-Dependent Protection Against Free Radical Formation and Oxidative Stress in Skin Treated with Cumene Hydroperoxide

Details

• Personal Author:
  Castranova, Vincent ; Gunther M ; Kadiiska M ; Kisin E ; Mason R ; Murray A ; Shvedova A
• Description:
  Organic peroxides, widely used in the chemical and pharmaceutical industry are considered to be one of the key factors contributing to skin tumor promotion via free radical production. In vitro experiments have demonstrated metal-catalyzed formation of alkoxyl, alkyl, and aryl radicals in keratinocytes incubated with cumene hydroperoxide. The present study investigated in vivo free radical generation in lipid extracts of mouse skin exposed to cumene hydroperoxide. The ESR spin-trapping was used to detect the formation of alpha-phenyl-N-tert-butylnitrone (PBN) radical adducts, following intradermal Injection of 180 mg/kg PBN. After topical exposure (30 min), cumene hydroperoxide (12 mmole/kg) induced free radical generation in the skin of female Balb/c mice kept for 10 weeks on vitamin E-deficient diets. In contrast, hardly discernible radical adducts were detected when cumene hydroperoxide was applied to the skin of mice fed a vitamin E-sufficient diet. Total antioxidant reserve, levels of GSH, ascorbate and vitamin E decreased 34%, 46.5% 27% and 98%, respectively, after mice were kept for 10 weeks on vitamin E-deficient diet. PBN adducts detected by ESR in vitamin E- deficient mice provide direct evidence for in vivo free radical generation in the skin after exposure to cumene hydroperoxide. [Description provided by NIOSH]
• Subjects:
  Free Radicals Occupational Health Peroxides Safety Skin Diseases
• Keywords:
  Cell-growth Cell-morphology Free-radical-generation Free-radicals Humans Organic-peroxides Skin-disorders Skin-tumors Vitamins
• ISSN:
  0891-5849
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  North Carolina ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  33
• NIOSHTIC Number:
  nn:20032306
• Citation:
  Free Radic Biol Med 2002 Nov; 33(Suppl 2):S339
• CAS Registry Number:
  Cumene hydroperoxide (CAS RN 80-15-9)
• Federal Fiscal Year:
  2003
• Peer Reviewed:
  False
• Source Full Name:
  Free Radical Biology and Medicine
• Supplement:
  2
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:75497c3d26b7ffe55f9f5f5c5697b35ac4900ad142b129653b9d265adda05b4e5bd2533c8c29bf2e6e430eeb834e12b24a34e55f38fffe4feb17f76b4b6468b7
• Download URL:
  https://stacks.cdc.gov/view/cdc/186330/cdc_186330_DS1.pdf
• File Type:
  [PDF - 234.31 KB ]