Brief 95% O2 Exposure Effects on Surfactant Protein and mRNA in Rat Alveolar and Bronchiolar Epithelium

Details

• Personal Author:
  Allred TF ; Auten RL ; Deng H ; Mercer RR ; Thomas, Ronald F.
• Description:
  In acute lung injury, a disturbed surfactant system may impair gas exchange. Previous evaluations of hyperoxia effects on surfactant proteins (SPs) followed exposures >1-2 days. To evaluate the effects of brief exposure to hyperoxia on the SP system, we exposed adult male rats to 95% O2 or air for 12, 36, and 60 h. SP-A, -B, and -C mRNAs were analyzed by Northern blot and semiquantitative in situ hybridization (ISH). SP-A and -B were analyzed in whole lung homogenates, lung lavage fluid, and fixed tissue by semiquantitative immunohistochemistry (IHC). All SP mRNAs were diminished at 12 h and rose to or exceeded control by 60 h as determined by Northern blot and ISH. These effects were seen mainly in the intensity of ISH signal per cell in both type II and bronchiolar epithelial (Clara) cells and to a lesser extent on numbers of positively labeled cells. SP-B declined to 50% of control in lavage at 12 h, but no changes in total lung SP-A and -B were seen. The number of SP-A positively labeled cells did not change, but SP-A label intensity measured by IHC in type II cells showed parallel results to Northern blots and ISH. The response of SP-A in Clara cells was similar. SP-B immunolabeling intensity rose in both type II and Clara cells throughout the exposure. SP-C ISH intensity fell at 12 h and was increased to two times control by 60 h of hyperoxia. Sharp declines in SP expression occurred by 12 h of 95% O2 and may affect local alveolar stability. [Description provided by NIOSH]
• Subjects:
  Laboratories Lung Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Author Keywords: Lung Injury Hyperoxia In Situ Hybridization Laboratory-animals Oxygen-toxicity Pulmonary-system Pulmonary-system-disorders Respiratory-system-disorders Statistical-analysis
• ISSN:
  1040-0605
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  276
• Issue:
  6
• NIOSHTIC Number:
  nn:20027396
• Citation:
  Am J Physiol Lung Cell Mol Physiol 1999 Jun; 276(6):L999-L1009
• Contact Point Address:
  Thomas F. Allred, Division of Neonatal Medicine, Department of Pediatrics, Neonatal-Perinatal Research Institute, Duke University Medical Center, Durham, North Carolina 27710
• CAS Registry Number:
  Oxygen (CAS RN 7782-44-7)
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  True
• Source Full Name:
  American Journal of Physiology: Lung Cellular and Molecular Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:58a6ae5cf238515844a982be809cb5f7cfc9c35af7ed17ff2c30404acbc435fd09a2254b2bb3d2aeb38bc2fa2c27abeddd0ec4aa15abc9eab92017ebfc96529c
• Download URL:
  https://stacks.cdc.gov/view/cdc/197561/cdc_197561_DS1.pdf
• File Type:
  [PDF - 762.30 KB ]