Brief Exposure to 95% Oxygen Alters Surfactant Protein D and mRNA in Adult Rat Alveolar and Bronchiolar Epithelium

Details

• Personal Author:
  Aderibigbe AO ; Auten RL Jr. ; Mercer RR ; Thomas, Ronald F.
• Description:
  Surfactant protein D (SP-D), which has structural homology to C-type lectin binding regions, may play a role in host defense and has no known surfactant function. Because other surfactant proteins have been shown to be increased after prolonged periods of hyperoxia, we sought to evaluate the early effects of hyperoxia (95% O2) on expression of SP-D in the adult male rat lung. Animals were exposed to air or to 12, 36, or 60 h of 95% O2. Northern blot analysis of total lung RNA revealed marked SP-D mRNA increases at 12 h 95% O2 compared with air-exposed controls, with decreasing expression to near that of air-exposed animals by 60 h. Semiquantitative in situ RNA hybridization demonstrated parallel results, with increased numbers of labeled alveolar epithelial (AE) and bronchiolar epithelial (BE) cells at 12 h and increased intensity of labeled alveolar cells, compared with air-exposed controls. After 60 h of exposure to 95% O2, mRNA label intensity in AE and BE was decreased to levels near those seen in air-exposed animals. In contrast, Western blotting showed a decline in total lung SP-D with 95% O2 exposure, beginning at 12 h and continuing at 36 and 60 h, respectively. Semiquantitative immunohistochemistry demonstrated a decline in AE labeling parallel to the total lung Western blot results, but labeled total BE cell numbers increased (P = 0.10). Hyperoxia had differential effects on SP-D abundance in AE and BE cells, and therefore may influence the availability of SP-D to bind microbial pathogens in the airways depending on cell type and location. [Description provided by NIOSH]
• Subjects:
  Environmental Monitoring Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Exposure-assessment Laboratory-animals Lung-disorders Oxygen-toxicity Pulmonary-system Pulmonary-system-disorders Respiratory-system-disorders Statistical-analysis
• ISSN:
  1044-1549
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  219-227
• Volume:
  20
• Issue:
  2
• NIOSHTIC Number:
  nn:20027395
• Citation:
  Am J Respir Cell Mol Biol 1999 Feb; 20(2):219-227
• Contact Point Address:
  R. L. Auten, DUMC Box 3179, Durham, NC 27710
• Email:
  auten@acpub.duke.edu
• CAS Registry Number:
  Oxygen (CAS RN 7782-44-7)
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  True
• Source Full Name:
  American Journal of Respiratory Cell and Molecular Biology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:bd4475f15fee4db306eba35793f19a380dccb4a53b775d7bceba64699c525f4e39ffa8241aaaaab121cb6c054fc41afc02ba4d8cee28547a69d3c3173233eebb
• Download URL:
  https://stacks.cdc.gov/view/cdc/197560/cdc_197560_DS1.pdf
• File Type:
  [PDF - 666.83 KB ]