Free Radical Scavenging, DNA Protection, and Inhibition of Lipid Peroxidation Mediated by Uric Acid

Details

• Personal Author:
  Blemings KP ; Klandorf H ; Leonard, Stephen S. ; Shi X ; Stinefelt B
• Description:
  Uric acid (UA) has been proposed to be the dominant antioxidant in birds. The objective of this study was to investigate the quenching effect of varying concentrations of UA, including those found in avian plasma, on specific reactive oxygen species (ROS) and to determine the ability of UA to protect DNA and cellular membranes from ROS-mediated damage. Hydroxyl (OH) and superoxide (O2) radicals were detected by electron spin resonance (ESR) and their presence was reduced following addition of UA (p <0.05) in a concentration-dependent manner. UA inhibited hydroxyl-mediated DNA damage, indicated by the presence of more precise, dense bands of Hind III DNA after agarose gel electrophoresis and ethidium bromide staining (p <0.05). Lipid peroxidation of silica-exposed RAW 264.7 cell membranes was diminished (p <0.02) after addition of UA to the cell incubation mixture. These studies demonstrate that UA scavenges hydroxyl and superoxide radicals and protects against DNA damage and lipid peroxidation. These results indicate specific antioxidant protection that UA may afford birds against ROS-mediated damage. [Description provided by NIOSH]
• Subjects:
  Aluminum Silicates Antioxidants DNA Free Radicals Lipids Occupational Health Safety Silicon Dioxide
• Keywords:
  Author Keywords: Uric Acid DNA-damage DNA Oxidation Electron Spin Resonance Exposure-levels Free-radical-generation Free-radicals Hydroxyl-groups Lipid-peroxidation Lipid Peroxidation Oxidative Stress Silica-dusts Silicates

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• ISSN:
  0091-7370
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  37-45
• Volume:
  35
• Issue:
  1
• NIOSHTIC Number:
  nn:20026395
• Citation:
  Ann Clin Lab Sci 2005 Jan; 35(1):37-45
• Contact Point Address:
  Kenneth P. Blemings, Ph.D., West Virginia University, P.O. Box 6108, Morgantown, WV 26506, USA
• Email:
  kbleming@wvu.edu
• Federal Fiscal Year:
  2005
• NORA Priority Area:
  Research Tools and Approaches: Cancer Research Methods
• Peer Reviewed:
  True
• Source Full Name:
  Annals of Clinical and Laboratory Science
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:435e4b4fffd38a67c3bf3602121c621b7cea0d54010783970642485c655de14ddd58d2a9560d3659c3cd3d8c1dc2148be9e48d57173e741ceeff21a66e117480
• Download URL:
  https://stacks.cdc.gov/view/cdc/197268/cdc_197268_DS1.pdf
• File Type:
  [PDF - 3.14 MB ]