Development of a Sensitivity Enhanced Multiplexed Fluorescence Covalent Microbead Immunosorbent Assay (FCMIA) for the Measurement of Glyphosate, Atrazine and Metolachlor Mercapturate in Water and Urine

Details

• Personal Author:
  Biagini, Raymond E. ; MacKenzie, Barbara A. ; Robertson SK ; Sammons DL ; Smith JP ; Snawder, John E. ; Striley CA
• Description:
  Body burdens from exposures to pesticides may be estimated from urinary analyses of pesticide parent/metabolite concentrations. Pesticide applicators and others are often exposed to numerous unrelated pesticides, either sequentially or simultaneously. Classically, body burdens of pesticides are analyzed using chemical/instrumental analysis (CIM) or enzyme immunoassays (EIAs). Both of these technologies can usually be used to quantitate one analyte (or closely related groups of analytes) per analysis. Alternatively, multiple analytes can be measured simultaneously using a multiplexed fluorescence covalent microbead immunoassay (FCMIA). We developed a multiplexed FCMIA to simultaneously measure glyphosate (Gly), atrazine (Atz), and metolachlor mercapturate (MM) in water and urine. The assay had least detectable doses (LDDs) in water/diluted urine of 0.11/0.09 ng/ml (Gly, water/urine LDD), 0.10/0.07 ng/ml (Atz) and 0.09/0.03 ng/ml (MM). The sensitivity for the measurement of Gly was enhanced by derivatization. All assays gave linear responses from the LDDs for each respective pesticide to 300 ng/ml. There was no cross-reactivity between the three analytes. Using a 96-well microplate and an autosampler, as many as 288 separate analyses can be completed in approximately 120 min with precision, sensitivity, and specificity equivalent to, if not better, than that found when these same analytes are measured by CIM or EIA. [Description provided by NIOSH]
• Subjects:
  Chemistry Chemistry, Analytic Insecticides Occupational Health Safety Urine
• Keywords:
  Analytical-chemistry Analytical-methods Analytical-processes Atrazine Author Keywords: Biomonitoring Glyphosate; Insecticide-poisoning Luminex Metolachlor Mercapturate Pesticides Pesticides-and-agricultural-chemicals Urinalysis

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• ISSN:
  1618-2642
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  368-374
• Volume:
  379
• Issue:
  3
• NIOSHTIC Number:
  nn:20026130
• Citation:
  Anal Bioanal Chem 2004 Jun; 379(3):368-374
• Contact Point Address:
  Biomonitoring and Health Assessment Branch, Division of Applied Research and Technology, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 4676 Columbia Parkway, Cincinnati, OH 45226
• Email:
  reb4@cdc.gov
• Federal Fiscal Year:
  2004
• Peer Reviewed:
  True
• Source Full Name:
  Analytical and Bioanalytical Chemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c0a803fb61bb9dbc2ee70d9e0f5df37edc4843b73f3c46ddbb86535fd5d9747b09bd9cfec6d0ea8e68be0d9d3364afa3982aec089140692f3d8a930df441b578
• Download URL:
  https://stacks.cdc.gov/view/cdc/197200/cdc_197200_DS1.pdf
• File Type:
  [PDF - 268.06 KB ]