Development of an Enzyme-Linked Immunosorbent Assay for the Pyrethroid Cypermethrin

Details

• Personal Author:
  Ahn KC ; Gee SJ ; Hammock BD ; Lee H-J ; Park E-K ; Shan G ; Watanabe T
• Description:
  A competitive enzyme-linked immunosorbent assay (ELISA) for the detection of cypermethrin was developed. Two haptens, the trans- and cis-isomers of 3-[(+/-)-cyano-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarbonyloxy]methyl]phenoxyacetic acid, were conjugated with thyroglobulin as immunogens. Four antisera were generated and screened against six different coating antigens. The assay that was the most sensitive for cypermethrin was optimized and characterized. The IC(50) for cypermethrin was 13.5 +/- 4.3 microg/L, and the lower detection limit (LDL) was 1.3 +/- 0.5 microg/L. This ELISA had relatively low cross-reactivities with other major pyrethroids, such as deltamethrin, phenothrin, resmethrin, fluvalinate, and permethrin. Methanol was found to be the best organic cosolvent for this ELISA, with an optimal sensitivity observed at a concentration of 40% (v/v). The assay parameters were unchanged at pH values between 5.0 and 8.0, whereas higher ionic strengths strongly suppressed the absorbances. To increase the sensitivity of the overall method, a C(18) sorbent-based solid-phase extraction was applied to various domestic and environmental water samples. The water samples, fortified with cypermethrin, were analyzed according to this method. Good recoveries and correlation with spike levels were observed. A competitive enzyme-linked immunosorbent assay (ELISA) for the detection of cypermethrin was developed. Two haptens, the trans- and cis-isomers of 3-[(+/-)-cyano-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarbonyloxy]methyl]phenoxyacetic acid, were conjugated with thyroglobulin as immunogens. Four antisera were generated and screened against six different coating antigens. The assay that was the most sensitive for cypermethrin was optimized and characterized. The IC(50) for cypermethrin was 13.5 +/- 4.3 microg/L, and the lower detection limit (LDL) was 1.3 +/- 0.5 microg/L. This ELISA had relatively low cross-reactivities with other major pyrethroids, such as deltamethrin, phenothrin, resmethrin, fluvalinate, and permethrin. Methanol was found to be the best organic cosolvent for this ELISA, with an optimal sensitivity observed at a concentration of 40% (v/v). The assay parameters were unchanged at pH values between 5.0 and 8.0, whereas higher ionic strengths strongly suppressed the absorbances. To increase the sensitivity of the overall method, a C(18) sorbent-based solid-phase extraction was applied to various domestic and environmental water samples. The water samples, fortified with cypermethrin, were analyzed according to this method. Good recoveries and correlation with spike levels were observed. [Description provided by NIOSH]
• Subjects:
  Antigens Chemistry, Analytic Enzymes Immune System Insecticides Occupational Health Safety
• Keywords:
  Analytical Chemistry Analytical Methods ELISA Enzyme-linked Immunosorbent Assay Immunology Immunotoxins Pesticides And Agricultural Chemicals
• ISSN:
  0021-8561
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  52
• Issue:
  5
• NIOSHTIC Number:
  nn:20029281
• Citation:
  J Agric Food Chem 2004 Mar; 52(5):1039-1043
• Email:
  bdhammock@ucdavis.edu
• CAS Registry Number:
  Ammonia (CAS RN 7664-41-7)
• Federal Fiscal Year:
  2004
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Journal of Agricultural and Food Chemistry
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2987de8ea3216d6da92dea008be55730c99b738b09a39d6cf923f68cbe0d66dca96a19b8abb327ffbeb1b4424be34d92ac52cad450695543156a59094f0b92ce
• Download URL:
  https://stacks.cdc.gov/view/cdc/190306/cdc_190306_DS1.pdf
• File Type:
  [PDF - 69.23 KB ]