Simultaneous Measurement of Bacillus thuringiensis Cry1Ab and Cry3B Proteins in Corn Extracts

Details

• Personal Author:
  Biagini, Raymond E. ; Ferguson BS ; Larkin KA ; MacKenzie, Barbara A. ; Robertson SA ; Sammons DL ; Smith JP ; Snawder, John E. ; Striley CAF
• Description:
  Transgenic com hybrids have been developed which contain synthetic genes for the production of Bacillus thuringiensis insecticidal proteins. Two of these proteins are Cry1Ab and Cry3B. Enzyme-linked immunosorbent assays (ELISA) and immunochromatographic lateral flow assays, which are sensitive, specific and easy to perform, have been developed for Cry1Ab and Cry3B proteins in corn extracts. In the present work, we describe, a sandwich fluorescent covalent microsphere immunoassay (FCMIA) to simultaneously measure Cry1Ab and Cry3B proteins in corn extracts. Mixtures of genetically modified organism (GMO) and nonGMO corn were prepared and extracted. The extracts were diluted and Cry1Ab and Cry3B were measured by FCMIA. Measurement of pure Cry1Ab protein by FCMIA gave a minimum detectable concentration of 0.1 ng/mL. There were no significant differences between the assays when performed singly vs. multiplexed (P>0.588). When the observed dilutions from the four parameter logistic fits were compared to the known dilutions, highly linear relationships were observed (r = 0.984 and 0.983, P<0.001) over a dilution range of 1 to 100. These data give proof of principle that FCMIA can be used to simultaneously measure multiple GMO proteins in com and may be a valuable adjunct to existing assays when the number of GMO pesticidal proteins to be measured becomes large. [Description provided by NIOSH]
• Subjects:
  Chemistry Chemistry, Analytic Genetics Insecticides Occupational Health Safety
• Keywords:
  Analytical-chemistry Analytical-processes Bacteria Genetic-engineering Pesticides
• ISBN:
  9780841274204
• Publisher:
  American Chemical Society
• Document Type:
  Text
• Genre:
  Book
• Place as Subject:
  Maine ; Ohio ; OSHA Region 1 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  274-285
• NIOSHTIC Number:
  nn:20032949
• Citation:
  Rational environmental management of agrochemicals: risk assessment, monitoring, and remedial action. Kennedy IR, Solomon KR, Gee SJ, Crossan AN, Wang S, Sanchez-Bayo-F, eds. Washington, DC: American Chemical Society, 2007 Aug; :274-285
• Contact Point Address:
  National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Biomonitoring and Health Assessment Branch, 4676 Columbia Parkway, Cincinnati, OH 45226
• Editor(s):
  Kennedy IR; Solomon KR; Gee SJ; Crossan AN; Wang S; Sanchez-Bayo F
• Federal Fiscal Year:
  2007
• NORA Priority Area:
  Agriculture, Forestry and Fishing ; Manufacturing ; Construction
• Peer Reviewed:
  False
• Source Full Name:
  Rational environmental management of agrochemicals: risk assessment, monitoring, and remedial action
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f39b193c2ed6e64027b652acdba2333dacc298c1ca46a0fd910753e20ab916d49496d7b464a213603a8e95e3ed8186b82b87877820e72f4d320a98fe266b7700
• Download URL:
  https://stacks.cdc.gov/view/cdc/188704/cdc_188704_DS1.pdf
• File Type:
  [PDF - 1.77 MB ]