Development of a Headspace Gas Chromatographic Test for the Quantification of 1- and 2-Bromopropane in Human Urine

Details

• Personal Author:
  B'Hymer, Clayton ; Cheever KL
• Description:
  A test procedure was developed for the detection and quantification of 1- and 2-bromopropane in human urine. 1-Bromopropane (1-BP) is a commonly used industrial solvent, and 2-bromopropane (2-BP) is often found as an impurity component in industrial grade 1-BP. Both compounds are a health concern for exposed workers due to their chronic toxicity. Bromopropanes have been associated with neurological disorders in both animals and humans. Sample preparation consisted of diluting urine with water and fortification with 1-bromobutane (1-BB), which was used as an internal standard; then each sample was sealed in a headspace vial. A static-headspace sampler (Teledyne-Tekmar Model 7000) was used to heat each sample at 75 degrees C for a 35-min equilibrium time. Quantification was by means of a gas chromatograph (GC) equipped with an electron capture detector (ECD) and a dimethylpolysiloxane (DB-1) capillary column. A recovery study using fortified urine samples at multiple concentrations (0.5-8 microg/ml) demonstrated full recovery; 104-121% recovery was obtained. Precision ranged from 5 to 17% for the 15-20 spiked samples used at each concentration, which were analyzed over multiple experimental trial days. The limit of detection (LOD) for this test procedure was approximately 2 ng/ml 1-BP and 7 ng/ml 2-BP in urine. A recovery study of 1- and 2-BP from fortified urine stored in vials appropriate for field collection was also completed. These results and other factors of the development and validation of this test procedure will be discussed. [Description provided by NIOSH]
• Subjects:
  Animals Hazardous Substances Nervous System Nervous System Diseases Occupational Health Safety Solvents Urine
• Keywords:
  2-Bromopropane Author Keywords: 1-Bromopropane Exposure-levels Gas-chromatography Health-hazards Humans Neurological-diseases Neurological-system Toxins Urinalysis Worker-health Workers

  More +
• ISSN:
  1570-0232
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  185-189
• Volume:
  814
• Issue:
  1
• NIOSHTIC Number:
  nn:20025924
• Citation:
  J Chromatogr B 2005 Jan; 814(1):185-189
• Contact Point Address:
  U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Taft Laboratory, 4676 Columbia Parkway, Cincinnati, OH 45226
• Email:
  cbhymer@cdc.gov
• CAS Registry Number:
  1-Bromopropane (CAS RN 106-94-5) ; 2-Bromopropane (CAS RN 75-26-3)
• Federal Fiscal Year:
  2005
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4b33ee4e674b071e8bd5a5ff9066b858760b6c30083897588107c079845e0fdc66a99eec21c550059b596abdc1b6f3e6eaba16a3e4daa0898d85d24bec9cccd7
• Download URL:
  https://stacks.cdc.gov/view/cdc/197092/cdc_197092_DS1.pdf
• File Type:
  [PDF - 76.64 KB ]