Urinary 3-Bromopropionic Acid: An Effective Gas Chromatographic Test Method for Quantification

Details

• Personal Author:
  B'Hymer, Clayton ; Cheever KL
• Description:
  3-Bromopropionic acid is a metabolite and possible biomarker for exposure to 1-bromopropane. 1-Bromopropane is used as an industrial solvent and exposure is a health concern for industrial workers due to its toxicity. Central neurological disorders and peripheral neuropathy has been reported in workers chronically exposed to materials composed of 1-bromopropane in the United States and animal studies have shown reproductive toxicity. Occupational exposure to this widely used industrial solvent is likely, since it is readily absorbed through the skin. A simple and effective general test method for 3-bromopropionic acid in urine samples was developed to monitor any exposed population. Urine specimens were first spiked with 3-chloropropionic acid which was used as a procedural internal standard. The samples were extracted with ethyl acetate, concentrated, and treated with N-methyl-N-[ tert-butyldimethylsilyl] trifluoroacetamide (MTBSTFA) to produce the corresponding t-butyldimethylsilane derivatives of 3-bromopropionic acid and the internal standard. Quantification was by means of a gas chromatograph (GC) equipped with a mass selective detector (MSD) using a 50-m X 0.20-mm (id) HP-1 capillary column. A temperature program of 60 to 255C was used for the gas chromatographic measurement. Ion m/z 211 was monitored for the derivative of 3-bromopropionic acid and ion m/z 165 was monitored for the internal standard. Average recovery of known 3-bromopropionic acid fortified blank urine samples was between 93-98% with relative standard deviations as high as 5.7% using samples at 3-bromopropionic concentrations of 2, 10, 20 and 50 ug/ml. The limit of detection (LOD) for the developed procedure was found to be approximately 0.01 ug/ml in urine. [Description provided by NIOSH]
• Subjects:
  Acids Animals Biomarkers Hazardous Substances Laboratories Nervous System Nervous System Diseases Occupational Exposure Occupational Health Safety Solvents

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• Keywords:
  Animal-studies Exposure-levels Gas-chromatography Health-hazards Laboratory-animals Neurological-diseases Occupational-exposure Occupational-hazards Sampling Skin-infections Toxic-effects Toxic-gases

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• ISSN:
  1096-6080
• Publisher:
  Society of Toxicology
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Maryland ; Ohio ; OSHA Region 3 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  78
• NIOSHTIC Number:
  nn:20025086
• Citation:
  Toxicologist 2004 Mar; 78(S-1):178
• CAS Registry Number:
  3-Bromopropionic acid (CAS RN 590-92-1)
• Federal Fiscal Year:
  2004
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 43nd Annual Meeting and ToxExpo, March 21-25, 2004, Baltimore, Maryland
• Supplement:
  S-1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c1128e914dc499da8d073a395442d03c6a6faa4241420fe975cba9ee478fa4ad967ce4d93e93b3462daf43c53c2bada9bd6b43d35e2c86ced8804702061f8b5d
• Download URL:
  https://stacks.cdc.gov/view/cdc/196587/cdc_196587_DS1.pdf
• File Type:
  [PDF - 57.49 KB ]