Selective Accumulation of 8-Hydroxy-2′-Deoxyguanosine in the Lung Following Acute Treatment with Benzo(a)pyrene

Details

• Personal Author:
  Cardozo-Pelaez F ; Harbison RD ; Hover C ; Sanchez-Ramos J ; Stedeford T
• Description:
  The lung has been shown to be a target organ for the effects of Benzo[a]pyrene (B[a]P). 8-hydroxy-2'-deoxyguanosine (oxo8dG) is a mutagenic oxidized base formed in DNA during the metabolism of B[a]P by the peroxidase and prostaglandin H synthetase systems. The objective of this study was to determine the capacity to cleave oxo8dG in the lung, liver, and kidney by measuring the activity of 8-oxoguanosine DNA glycosylase (Ogg1),and whether exposure to B[a]P had an effect in this DNA repair system. Male Sprague-Dawley rats were administered 20 mg/kg B[a]P i.p., 2 times/day for 5 days. A 26% decrease in the capacity to remove oxo8dG was observed in lung tissue at 72 hours and recovered 20% above control values at 120 hours. The capacity of the liver and kidney remained at baseline for all time points analyzed. A 7-fold increase in oxo8dG was observed in the lung at 72 hours and returned to basal levels by 120 hours. A statistically significant increase of Ogg1 protein levels was observed at 120 hours. Oxo8dG and Ogg1 protein levels were not significantly changed in the liver and kidney. This study demonstrates that organ-specific differences exist in the accumulation of oxo8dG and the capacity to remove this mutagenic base following i.p. treatment with B[a]P. [Description provided by NIOSH]
• Subjects:
  Animals Benzopyrenes DNA Environmental Monitoring Laboratories Lung Occupational Health Pyrenes Respiratory System Safety
• Keywords:
  Animal-studies DNA-damage Exposure-assessment Exposure-levels Kidneys Laboratory-animals Liver Lung-tissue
• ISSN:
  1096-6080
• Publisher:
  Oxford University Press
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Abstract or Summary
• Place as Subject:
  Florida ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  60
• Issue:
  1
• NIOSHTIC Number:
  nn:20025814
• Citation:
  Toxicologist 2001 Mar; 60(1):382
• CAS Registry Number:
  Benzo[a]pyrene (CAS RN 50-32-8)
• Federal Fiscal Year:
  2001
• Performing Organization:
  University of South Florida
• Peer Reviewed:
  False
• Start Date:
  19980930
• Source Full Name:
  The Toxicologist. Society of Toxicology 40th Annual Meeting, March 25-29, 2001, San Francisco, California
• End Date:
  20020929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8cd744cd2f7a51952c5468f4071b00d95be1be958095987980c901b8fc621038b66da2efea2e207a6e571272d896ab802f96fa207f95d315e41c7353c41b3a71
• Download URL:
  https://stacks.cdc.gov/view/cdc/197030/cdc_197030_DS1.pdf
• File Type:
  [PDF - 129.10 KB ]