Characterization of DNA Adducts from Lung Tissue of Asphalt Fume-Exposed Mice by Nanoflow Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry

Details

• Personal Author:
  Frazer DG ; Law B ; Lewis, Daniel M. ; Marshall WD ; Wang JJ
• Description:
  A bioanalytical method based on nanoflow liquid chromatography coupled to a hybrid quadrupole orthogonal acceleration time-of-flight mass spectrometry was developed to characterize selected polyaromatic hydrocarbon (PAH)-DNA adducts. The collision-induced dissociation of analytes results in characteristic fragmentation patterns that can be utilized to identify the DNA adducts. In the experiment, 32 B6C3F1 mice were exposed daily (4 h/day) to asphalt fume in a whole-body inhalation chamber for 10 days; 16 nonexposed mice served as controls. The asphalt fume was generated at 180 degrees C and the concentrations of PAHs in the animal exposure chamber ranged from 152 to 198 mg/m3. The DNA adducts N2-deoxyguanosine-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide (N2-dG-BPDE); N6-deoxyadenosine-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide (N6-dA-BPDE), and N4-deoxycytidine-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide (N4-dC-BPDE) were identified. The concentrations of N2-dG-BPDE, N6-dA-BPDE, and N4-dC-BPDE adducts were determined to be 1.17, 0.97, and 0.68 pmol/mg DNA, respectively, in the lung tissue of exposed mice using the nanoflow technique. The total DNA adducts in exposed lung tissue was determined to be 8.35 pmol/mg DNA by 32P-postlabeling assay. In total, the results indicated that PAH-DNA adducts were significantly elevated (p<0.001) in the lung tissue of asphalt-fume-exposed mice relative to tissue from control animals. [Description provided by NIOSH]
• Subjects:
  Animals Benzopyrenes Biological Assay Chromatography Environmental Monitoring Hydrocarbons Laboratories Lung Mass Spectrometry Occupational Health Polycyclic Aromatic Hydrocarbons Respiratory System Safety

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• Keywords:
  Analytical-methods Animal-studies Asphalt-fumes Bioassays Chromatographic-analysis DNA-adducts Exposure-assessment Exposure-levels Laboratory-animals Lung-tissue Mass-spectrometry Polycyclic-aromatic-hydrocarbons

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• ISSN:
  0003-2697
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  79-88
• Volume:
  322
• Issue:
  1
• NIOSHTIC Number:
  nn:20032136
• Citation:
  Anal Biochem 2003 Nov; 322(1):79-88
• Contact Point Address:
  National Institute for Occupational Safety and Health, Health Effects Laboratory Division, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  juw9@cdc.gov
• CAS Registry Number:
  Benzo[a]pyrene-7,8-dihydrodiol (CAS RN 13345-25-0) ; Polytetrafluoroethylene (CAS RN 9002-84-0)
• Federal Fiscal Year:
  2004
• Peer Reviewed:
  True
• Source Full Name:
  Analytical Biochemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/186184/cdc_186184_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]