A Test Procedure for the Determination of (2-Methoxyethoxy) Acetic Acid in Urine from Jet Fuel-Exposed Mice

Details

• Personal Author:
  B'Hymer, Clayton ; Cheever KL ; Keil DE
• Description:
  A test procedure for the determination of (2-methoxyethoxy)acetic acid (MEAA) was adapted and applied to urine samples from jet fuel (JP-8)-exposed mice using capillary gas chromatography with a mass selective detector (MSD). MEAA is a metabolite and proposed biomarker for exposure to 2-(2-methoxyethoxy)ethanol, a glycol ether component in the formulation of JP-8. The collected urine samples were spiked with deuterated butoxyacetic acid internal standard, and extracted with ethyl acetate, and esterified with ethanol and sulfuric acid, and the esters of the glycol ethers were extracted with methylene chloride. The chromatographic conditions used easily separate the MEAA ethyl ester from interferences within mouse urine. The application of this procedure to urine samples collected from mice demonstrated that MEAA was detectable after oral (2000 mg/kg) or dermal (50 u L) exposure for 7 days to JP-8 at levels as high as 8.5 or 6.5 u g/mL, respectively. This pilot demonstration indicated that total urinary MEAA was a viable biomarker for the two routes of JP-8 exposure in laboratory mice. [Description provided by NIOSH]
• Subjects:
  Acetamides Acids Aircraft Animals Biomarkers Environmental Monitoring Laboratories Occupational Health Safety Urine
• Keywords:
  2-Methoxyethoxy Acetic Acid Acetic-acids Animal-studies Author Keywords: GC-MS Exposure-assessment Exposure-levels Gas-chromatography Glycol Ethers Jet-engine-fuels Jet Fuel JP-8 Laboratory-animals MEAA Sampling Urinalysis

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• ISSN:
  1537-6516
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology ; OD - Office of the Director ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  367-373
• Volume:
  15
• Issue:
  5
• NIOSHTIC Number:
  nn:20028655
• Citation:
  Toxicol Mech Methods 2005 Sep; 15(5):367-373
• Contact Point Address:
  U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health; Division of Applied Research and Technology, Taft Laboratory, 4676 Columbia Parkway, Cincinnati, Ohio, 45226
• CAS Registry Number:
  Acetic acid (CAS RN 64-19-7)
• Federal Fiscal Year:
  2005
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology Mechanisms and Methods
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f163e147ac1581a9d3f859934a49d22e5b4a7f4a5949d480798205ddbbf66c69b64ac5008352205bf28505f73a5dbd5a72fbd292740f5a4282534e383ec13cc9
• Download URL:
  https://stacks.cdc.gov/view/cdc/189872/cdc_189872_DS1.pdf
• File Type:
  [PDF - 347.90 KB ]