Development of a Gas Chromatographic Test for the Quantification of the Biomarker 3-Bromopropionic Acid in Human Urine

Details

• Personal Author:
  B'Hymer, Clayton ; Cheever KL
• Description:
  An accurate and precise method was developed for the detection and quantification of 3-bromopropionic acid (3-BPA), a metabolite and biomarker for exposure to 1-bromopropane (1-BP). 1-BP is used as an industrial solvent and exposure is a health concern for industrial workers due to its toxicity. It has been associated with neurological disorders in both animals and humans. Urine sample preparation for the determination of 3-BPA consisted of liquid-liquid extraction (LLE) with ethyl acetate and silylation with N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide (MTBSTFA). Quantification was by means of a gas chromatograph (GC) equipped with a mass selective detector (MSD) using a dimethylpolysiloxane (HP-1) capillary column and 3-chloropropionic acid was used as an internal standard in the procedure. Demonstrated accuracy and precision during this method's validation was good; recovery varied between 93 and 98% with relative standard deviations (R.D.S.) of 5.7% or less. The limit of detection (LOD) for the procedure was approximately 0.01microg/ml 3-BPA in urine. These data and other factors of the development and validation of this test method will be discussed. [Description provided by NIOSH]
• Subjects:
  Biomarkers Energy Metabolism Hazardous Substances Nervous System Nervous System Diseases Occupational Health Safety Solvents Urine
• Keywords:
  Analytical-methods Brominated-compounds Brominated-propanes Gas-chromatography Metabolites Neurological-diseases Toxic-effects Urinalysis
• ISSN:
  1570-0232
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  361-366
• Volume:
  802
• Issue:
  2
• NIOSHTIC Number:
  nn:20024734
• Citation:
  J Chromatogr B 2004 Apr; 802(2):361-366
• Contact Point Address:
  National Institute for Occupational Safety and Health, Division of Applied Research and Technology, 4676 Columbia Parkway, MS C-26, Cincinnati, OH 45226
• Email:
  cbhymer@cdc.gov
• CAS Registry Number:
  1-Bromopropane (CAS RN 106-94-5) ; 3-Bromopropionic acid (CAS RN 590-92-1)
• Federal Fiscal Year:
  2004
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:eea87117f9737ffa3ad0508cb9c7d0916781fce5fe018ba2f3435aba2a07e73db8e842c5f8a317a4a2b48008f41f98a917926d36d4b531785a84d4da4dba3dcb
• Download URL:
  https://stacks.cdc.gov/view/cdc/196373/cdc_196373_DS1.pdf
• File Type:
  [PDF - 93.08 KB ]