Development of a Gas Chromatographic Test for the Quantitation of the Biomarker 2-Butoxyacetic Acid in Urine Samples

Details

• Personal Author:
  B'Hymer, Clayton
• Description:
  An accurate and precise method is developed and evaluated for the detection and quantitation of 2-butoxyacetic acid (2-BAA), a metabolite and biomarker for human exposure to 2-butoxyethanol. The solvent 2-butoxyethanol (2-BE) is extensively used in various industrial and domestic applications, and it is a health concern owing to its toxicity. Sample preparation consists of liquid-liquid extraction (LLE) of urine, then esterification of 2-BAA to produce the ethyl ester analog. The gas chromatographic conditions utilize a dimethyl polysiloxane phase (HP-1) capillary column and a mass spectrometer (MS) for detection of the analyte. Validation of this method includes a recovery study using fortified urine samples, which demonstrated good accuracy and precision; recovery varied between 100% and 102% of theory, with relative standard deviations of replicate samples at 2.8% and less. The detection limit of this method ranges from 0.005 to 0.015 µg/mL equivalent level of 2-BAA in urine. [Description provided by NIOSH]
• Subjects:
  Biomarkers Energy Metabolism Hazardous Substances Occupational Health Safety Solvents Urine
• Keywords:
  Analytical-methods Brominated-compounds Gas-chromatography Metabolites Toxic-effects Urinalysis
• ISSN:
  0021-9665
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  422-427
• Volume:
  45
• Issue:
  7
• NIOSHTIC Number:
  nn:20032527
• Citation:
  J Chromatogr Sci 2007 Aug; 45(7):422-427
• Contact Point Address:
  National Institute for Occupational Safety and Health, Division of Applied Research and Technology, 4676 Columbia Parkway, MS C-26, Cincinnati, OH 45226
• Email:
  cbhymer@cdc.gov
• CAS Registry Number:
  1-Bromopropane (CAS RN 106-94-5) ; 3-Bromopropionic acid (CAS RN 590-92-1)
• Federal Fiscal Year:
  2007
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  False
• Source Full Name:
  Journal of Chromatographic Science
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:90b01982ad47cb132bf15b05cd51c56f7eb71522f1ca6607926dd25cc34d0063c9824e0e0ba05c1f6ade55fb11cb9e64c81c44d91dad20d6e7a87be0ac47e2de
• Download URL:
  https://stacks.cdc.gov/view/cdc/186464/cdc_186464_DS1.pdf
• File Type:
  [PDF - 133.74 KB ]