Vanadium Alters Macrophage Interferon-Y-Interactions and -Inducible Responses

Details

• Personal Author:
  Cohen MD ; McManus TP ; Qu Q ; Schlesinger RB ; Yang Z ; Zelikoff JT
• Description:
  Vanadium (V) impairs host resistance overall, and the antimicrobial activity and function of several intracellular enzymes of macrophages (M) in particular. Mouse WEHI-3 M-like cells were exposed overnight to subcytotoxic concentrations of ammonium metavanadate (NH4 VO3) to determine whether this observed immunomodulation might be due, at least in part, to altered M-interactions with interferon-y (IFNy) or IFNy-inducible responses, i.e., increased reactive oxygen intermediate (ROI) production, Ca2+ ion influx, and surface Class II/Ia antigen expression. Binding studies performed at 22 degrees C indicated that V -treated cells had =50% fewer actively-binding receptors, but binding affinities 450-times greater than that of control receptors. At 4 degrees C, V-treated cells had 98% fewer functional receptors, but again higher (145-fold) affinities. IFNy-receptor complex internalization was unaffected by V, although significantly higher in cells incubated at temperatures which minimize uptake. ROI production in IFNy-stimulated V-treated (either NH4 V03 or V 2 O5 [vanadium pentoxide]) cells was decreased relative to spontaneous production, while control cells showed consistent increases due to IFNy priming. Vanadium also reduced the Ca2+ ion influx rate into stimulated cells without affecting final cell Ca2+ burdens. Although V did not affect IFNy-induced Ia expression, exposures resulted in increased numbers of Ia-bearing cells with lower maximal antigen densities than control cells. The results of this study show that V exposure may alter M-mediated functions by modifying cell interactions with IFNy and subsequent IFNy-dependent parameters. [Description provided by NIOSH]
• Subjects:
  Animals Laboratories Lung Diseases Occupational Health Respiratory Tract Diseases Safety Vanadium Compounds
• Keywords:
  Ammonium-compounds Animal-studies Laboratory-animals Lung-disorders Pulmonary-system-disorders Respiratory-system-disorders Vanadium-compounds
• ISSN:
  0731-9193
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  15
• Issue:
  1
• NIOSHTIC Number:
  nn:20024497
• Citation:
  Toxicologist 1995 Mar; 15(1):257
• CAS Registry Number:
  Vanadium (CAS RN 7440-62-2)
• Federal Fiscal Year:
  1995
• NORA Priority Area:
  Other Occupational Concerns
• Performing Organization:
  New York University, New York, New York
• Peer Reviewed:
  False
• Start Date:
  19930501
• Source Full Name:
  The Toxicologist. Society of Toxicology 34th Annual Meeting, March 5-9,1995, Baltimore, Maryland
• End Date:
  19960430
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1e96aa9f6a5f948f1d367c96b394b3b4b51cc1855cea98513647b5a4cabd6754c0247480145cfb2b541d9685b79eb10474faf88f65a0f712fa21a8d74d702e48
• Download URL:
  https://stacks.cdc.gov/view/cdc/196272/cdc_196272_DS1.pdf
• File Type:
  [PDF - 132.82 KB ]