Investigation of Protein Expression in Magnetic Field-Treated Human Glioma Cells

Details

• Personal Author:
  Conover, David L. ; Kanitz MH ; Lotz, W. Gregory ; Savage RE Jr. ; Witzmann FA
• Description:
  We previously reported phenotypic changes in human breast cancer cells following low-level magnetic field (MF) exposure. Here proteomic methods were used to investigate the biochemical effect of MF exposure in SF767 human glioma cells. Protein alterations were studied after exposure to 1.2 microTesla (µT) MF [12 milliGauss (mG), 60 Hertz (Hz)] +/- epidermal growth factor (EGF). SF767 cells were exposed for 3 h to sham conditions (<0.2 µT ambient field strength) or 1.2 µT MF (+/-EGF; 10 ng/ml). Solubilized protein fractions (sham; 1.2 µT; sham + EGF; 1.2 µT + EGF) were loaded for electrophoresis by 2D-PAGE and stained using a colloidal Coomassie blue technique to resolve and characterize the proteins. Protein patterns were compared across groups via Student's t-test using PDQUEST software. Cell profiles revealed significant alterations in the spot density of a subset of treated cells. Automated spot excision and processing was performed prior to peptide mass fingerprinting proteins of interest. Fifty-seven proteins from the detectable pool were identified and/or found to differ significantly across treatment groups. The mean abundance of 10 identified proteins was altered following 1.2 µT exposure. In the presence of EGF six proteins were altered after low magnetic field treatment by increasing (4) or decreasing (2) in abundance. The results suggest that the analysis of differentially expressed proteins in SF767 cells may be useful as biomarkers for biological changes caused by exposure to magnetic fields. [Description provided by NIOSH]
• Subjects:
  Biomarkers Carcinogens Genetics Magnetic Fields Occupational Health Safety
• Keywords:
  Author Keywords: ELF Magnetic Field Carcinogenesis Carcinogenicity Cell-function Exposure-levels Genes Genetic-factors Glioma Magnetic-fields Protein Expression Proteins Proteomics Two-dimensional Gel Electrophoresis

  More +
• ISSN:
  0197-8462
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Indiana ; Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  28
• Issue:
  7
• NIOSHTIC Number:
  nn:20032810
• Citation:
  Bioelectromagnetics 2007 Oct; 28(7):546-552
• Contact Point Address:
  M.H. Kanitz, MGMT, BHAB, DART, NIOSH, 4676 Columbia Parkway, MS/C-23, Cincinnati, OH 45226-1998
• Email:
  mhk2@cdc.gov
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  True
• Source Full Name:
  Bioelectromagnetics
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2b75ba5a104d3ae1f28197d07a389baf58ff74ce688abed66253166c49f4d67603a4ac23b433ad7a3e65365d6f2b4d9dfea43ac361c77f8d5b385152c0c8a2da
• Download URL:
  https://stacks.cdc.gov/view/cdc/186637/cdc_186637_DS1.pdf
• File Type:
  [PDF - 130.53 KB ]