Retinoid Signaling in Immortalized and Carcinoma-Derived Human Uroepithelial Cells

Details

• Personal Author:
  Benbrook DM ; Dhar A ; Gordon N ; Hemstreet GP 3rd ; Hurst RE ; Waliszewska M ; Waliszewski P
• Description:
  This paper investigates the presence and functionality of retinoid signaling pathways in human urinary bladder carcinoma and SV40-immortalized uroepithelial cell lines. Only two of eight cell lines were proliferation-inhibited by 10 microM of either all-trans or 13-cis-retinoic acid. Transactivation of the CAT gene under control of a retinoid-responsive element demonstrated functionality of the signaling pathway in both sensitive cell lines and four of six resistant cell lines. Relative RT-PCR analysis of a panel of retinoid-responsive and inducible genes demonstrated changes in expression levels of all the genes in response to-retinoic acid treatment together with numerous aberrations dysregulations. We conclude that retinoid signaling may be a target for inactivation during tumorigenesis by uncoupling gene expression, proliferation and differentiation. Therefore retinoids are more likely to be effective for chemoprevention than for treatment of bladder carcinomas. [Description provided by NIOSH]
• Subjects:
  Biomarkers Carcinogens Environmental Monitoring Neoplasms Occupational Health Risk Assessment Risk Factors Safety Urogenital Diseases Urogenital System
• Keywords:
  Bladder-cancer Bladder-disorders Cancer Carcinogenesis Carcinogenicity Chemotherapy Exposure-assessment Risk-analysis Risk-factors Tumorigenesis Tumorigens Urogenital-system-disorders

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• ISSN:
  0303-7207
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Oklahoma ; OSHA Region 6
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  55-65
• Volume:
  148
• Issue:
  1
• NIOSHTIC Number:
  nn:20031355
• Citation:
  Mol Cell Endocrinol 1999 Feb; 148(1-2):55-65
• Contact Point Address:
  Department of Urology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA
• Email:
  robert-hurst@ouhsc.edu
• Federal Fiscal Year:
  1999
• Performing Organization:
  University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma
• Peer Reviewed:
  True
• Start Date:
  19890930
• Source Full Name:
  Molecular and Cellular Endocrinology
• End Date:
  19990430
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c1c7ecd00b17ec8fdea7bf20fd68a77b65b48ed51a1f8fb8ba8938c2832c109e822d24d24e11575aac15c31d3fc957fa3de3780af9e54251a10505ebb0787c61
• Download URL:
  https://stacks.cdc.gov/view/cdc/191623/cdc_191623_DS1.pdf
• File Type:
  [PDF - 238.79 KB ]