Retinoid Signaling in Immortalized and Carcinoma-Derived Human Uroepithelial Cells

Details

• Personal Author:
  Benbrook DM ; Dhar A ; Gordon N ; Hemstreet GP 3rd ; Hurst RE ; Waliszewska M ; Waliszewski P
• Description:
  This paper investigates the presence and functionality of retinoid signaling pathways in human urinary bladder carcinoma and SV40-immortalized uroepithelial cell lines. Only two of eight cell lines were proliferation-inhibited by 10 microM of either all-trans or 13-cis-retinoic acid. Transactivation of the CAT gene under control of a retinoid-responsive element demonstrated functionality of the signaling pathway in both sensitive cell lines and four of six resistant cell lines. Relative RT-PCR analysis of a panel of retinoid-responsive and inducible genes demonstrated changes in expression levels of all the genes in response to-retinoic acid treatment together with numerous aberrations dysregulations. We conclude that retinoid signaling may be a target for inactivation during tumorigenesis by uncoupling gene expression, proliferation and differentiation. Therefore retinoids are more likely to be effective for chemoprevention than for treatment of bladder carcinomas. Description provided by NIOSH
• Subjects:
  • Biomarkers
  • Carcinogens
  • Environmental Monitoring
  • Neoplasms
  • Occupational Health
  • Risk Assessment
  • Risk Factors
  • Safety
  • Urogenital Diseases
  • Urogenital System
• Keywords:
  • Risk-factors
  • Risk-analysis
  • Cancer
  • Chemotherapy
  • Tumorigens
  • Tumorigenesis
  • Bladder-cancer
  • Biomarkers
  • Carcinogens
  • Carcinogenesis
  More +
• ISSN:
  0303-7207
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Oklahoma ; OSHA Region 6
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  55-65
• Volume:
  148
• Issue:
  1
• NIOSHTIC Number:
  nn:20031355
• Citation:
  Mol Cell Endocrinol 1999 Feb; 148(1-2):55-65
• Contact Point Address:
  Department of Urology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA
• Email:
  robert-hurst@ouhsc.edu
• Federal Fiscal Year:
  1999
• Performing Organization:
  University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma
• Peer Reviewed:
  True
• Start Date:
  19890930
• Source Full Name:
  Molecular and Cellular Endocrinology
• End Date:
  19990430
• Download URL:
  https://stacks.cdc.gov/view/cdc/191623/cdc_191623_DS1.pdf
• File Type:
  [PDF - 238.79 KB]
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c1c7ecd00b17ec8fdea7bf20fd68a77b65b48ed51a1f8fb8ba8938c2832c109e822d24d24e11575aac15c31d3fc957fa3de3780af9e54251a10505ebb0787c61