Determination of 3-Amino-5-Mercapto-1,2, 4-Triazole in Serum

Details

• Personal Author:
  Depree GJ ; Siegel, Paul D.
• Description:
  A high performance liquid chromatography (HPLC) method using fluorescence detection to determine 3-amino-5-mercapto-1,2,4-triazole (AMT) levels in serum has been developed. Sample preparation involved treatment with tributylphosphine (TBP) to reduce disulfides formed during storage, precipitation of proteins with acetonitrile (ACN), and precolumn derivatization using the thiol reactive fluorescent probe monobromobimane (MBB). The conjugate (AMT-MBB) was resolved by gradient elution from a C(18) reversed-phase column. The assay method was linear over a concentration range of 0.78-50 microg/ml and had a limit of detection (LOD) of 0.05 microg/ml AMT (10 microl injection). This method provides a sensitive and specific tool for the determination of AMT in serum and may have potential industrial hygiene application. [Description provided by NIOSH]
• Subjects:
  Antioxidants Chemistry Chemistry, Analytic Environmental Exposure Occupational Exposure Occupational Health Safety
• Keywords:
  Analytical-chemistry Analytical-methods Author Keywords: 3-Amino-5-mercapto-1,2,4-triazole Environmental-exposure Industrial-hygiene Occupational-exposure Proteins Sampling Sampling-methods
• ISSN:
  1570-0232
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  359-362
• Volume:
  801
• Issue:
  2
• NIOSHTIC Number:
  nn:20024352
• Citation:
  J Chromatogr B 2004 Mar; 801(2):359-362
• Contact Point Address:
  Analytical Services Branch, M/S L4218, 1095 Willowdale Road, Morgantown, WV 26505-2888, USA
• Email:
  pds3@cdc.gov
• CAS Registry Number:
  Acetonitrile (CAS RN 75-05-8) ; Tributylphosphine (CAS RN 998-40-3)
• Federal Fiscal Year:
  2004
• NORA Priority Area:
  Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a784650189194153c7880f8f8304264222e083609370d84963c103ccc8da5172714d269134db32dcce9df44b62ac2a59e5ac3d0c4f01cb7cfe34ce000d031df1
• Download URL:
  https://stacks.cdc.gov/view/cdc/196200/cdc_196200_DS1.pdf
• File Type:
  [PDF - 72.25 KB ]